天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (5): 867-872.doi: 10.16333/j.1001-6880.2017.5.026

• 开发研究 • 上一篇    下一篇

柚子皮精油提取及其驱蚊效果研究

杨愈丰1,2,3,柳敦耀1,3,向红英1,3,吕延成1,王菊芳2*   

  1. 1 遵义医学院珠海校区生物化学与分子生物学教研室,珠海519041;2 华南理工大学生物科学与工程学院,广州 510640;3珠海市中药基础与应用研究重点实验室,珠海519041
  • 出版日期:2017-05-31 发布日期:2017-06-09

Extraction and Mosquito Repellent Activity of Essential Oil from Citrus maxima (Burm.) Merr. cv. Shatian Yu peels

YANG Yu-feng1,2,3,LIU Dun-yao1,3,XIANG Hong-ying1,3,LV Yan-cheng1,WANG Ju-fang2*   

  1. 1 Department of Biochemistry and Moleculuar Biology,Zhuhai Campus of Zunyi Medical University,Zhuhai 519041,China;2School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510640,China;3 Zhuhai Key Laboratory of Fundamental and Applied Research in Traditional Chinese Medicine,Zhuhai Campus of Zunyi Medical University,Zhuhai 519041,China
  • Online:2017-05-31 Published:2017-06-09

摘要: 本文通过响应面法研究了柚子皮精油的提取工艺,并初步考察了所提取精油的驱蚊效果。柚子皮精油提取采用微波辅助蒸馏法,在采用单因素实验考察蒸馏时间、料液比和NaCl质量分数对精油提取率的影响之后,通过响应面法进行提取工艺优化,以获得最佳提取方案;然后采用趋避法验证柚子皮精油的驱蚊活性。结果表明,最佳柚子皮精油提取方案如下:蒸馏时间100 min,料液比0.3:2(g/mL),NaCl的质量分数1%,采用该方案柚子皮精油提取率达1.65%。通过初步驱蚊实验发现柚子皮精油具有较好的驱蚊活性,和市售六神花露水效果相当,驱蚊效应具有进一步深入研究的价值。

关键词: 柚子皮精油, 蒸馏法, 响应面法, 驱蚊活性

Abstract: The essential oil was extracted from Citrus maxima (Burm.) Merr. cv. Shatian Yu peels with distillation method. Response surface method was used to optimize the extraction process based on single factor experiments. Then the mosquito repellent activity of essential oil was tested by phobotaxis. The optimal extraction process were determined as follows:30 g of pomelo peel was treated in 200 mL of buffer containing 1% of sodium chloride for 100 min,of which the extraction yield was up to 1.65%. The extracted essential oil has considerable mosquito repellent activity,which deserve to be researched further. The result of this study provided scientific basis for the application of C. maxima essential oil.

Key words: pomelo peels essential oil, distillation, response surface method, mosquito repellent activity