关键词: 裂殖酵母, 多球壳菌素, 时序寿命, 衰老, 衰老相关疾病

Abstract: Looking for anti-aging compounds and studying its action mechanismare the focus and hotspot of aging pharmacology.Previous studies had found that the natural product of myriocin (a sphingolipid synthesis inhibitor) can prolong the lifespan of model organism budding yeast Saccharomyces cerevisiae, while the fission yeast Schizosaccharomycepombe is another modelorganism evolutionarily closer to the mammals,and is significantly different from the budding yeast in morphology and genetics.The effect of myriocin on the lifespan of fission yeast S.pombe was investigated in this study,and the mechanism of myrioin-mediated lifespan regulation was further studied.The results showed that myriocin conservatively prolonged lifespan of fission yeast S.pombe, and mechanisms for myrioin-mediated lifespan extension included enhancing cell stress resistance,accumulation of glycogen and trehalose,reducing intracellular reactive oxygen species.Further studies showed that myriocin-mediated lifespan extension depends on the stress-responsive protein kinase Sty1.In summary,myriocin is a potential anti-aging drug molecule,in future it is expected to be developed into a new drug for the delay of mammalian cells (including humans) aging and prevention and treatment of aging-related diseases.

Key words: fission yeast, myriocin, chronological lifespan, aging, age-related diseases