关键词: 银杏, 黄酮, 银杏酸, 多糖, 联合提取

Abstract: Petroleum ether,80% of ethanol and water were respectively used as solvent forintegrated extraction of ginkgo acid,flavone and polysaccharide from Ginkgo biloba sarcotesta successively.The result showed that: under the ambient temperature conditions,the cytoderm of Ginkgo biloba sarcotesta had been broken by high-speed shearing,petroleum ether was used to extract ginkgo acid for 40 min,two-time accumulative extraction rate reached 98.5%,the content reached 54.6%.Its purity after purification with silica gel column chromatography reached 97.5%;then 80% ethanol was used to extract flavonefor 60 min from residue,solid-to-liquid ratio was 1:15,the one-timeextraction rate reached 94.6%,the content of total flavone was 7.5%,the content of flavonoid glycoside was 24.4% after water precipitation and purification with macroporous resin column chromatography;at last,water was used as solvent to reflux extract the rest residue for 120 min under 90 ℃,solid-to-liquid ratio was 1:10,two-time accumulative extraction rate of polysaccharide reached 72.7%,the content was 24.3%,the purity reached 64% after purification by removing protein.

Key words: Ginkgo biloba, flavone, ginkgo acid, polysaccharide, combined extraction