天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (增刊1): 198-201.doi: 10.16333/j.1001-6880.2017.S.040

• 开发研究 • 上一篇    下一篇

复方多花黄精中多糖和荷叶碱水提工艺的研究

刘跃钧1*,温慧萍2,蒋燕锋1,戴德雄3,谢建秋1,彭小博1,朱虹1,葛永金1   

  1. 1丽水市林业科学研究院;2丽水学院;3浙江维康药业股份有限公司,丽水 323000
  • 出版日期:2017-08-02 发布日期:2017-08-02

Extraction Technology for Rhizoma Polygonati Polysaccharide and Nuciferine from Compound Polygonatum Cyrtonema

LIU Yue-jun1*,WEN Hui-ping2,JIANG Yan-feng1,DAI De-xiong3,XIE Jiang-qiu1,PENG Xiao-bo1,ZHU Hong1,GE Yong-jin1   

  1. 1 Lishui Academy of Forestry;2Lishui University;3Zhejiang Weikang Pharmacyeutical Industry Co.,Ltd.,Lishui 323000,China
  • Online:2017-08-02 Published:2017-08-02

摘要: 为了研究探讨复方多花黄精中多糖和荷叶碱的提取方法和工艺,试验采用3因素3水平正交设计方案考察了水提对多糖和荷叶碱含量的影响,结果表明:复方多花黄精中,多糖含量水提最佳工艺为:干药材粉碎50目→浸泡30 min→加热回流水提2 h,在此工艺条件下,250 g干药材粉,可得干浸膏54.81 g,多糖得率为64.36%,为总体平均得率的1.15倍;荷叶碱含量水提最佳工艺为:100目药材粉→浸泡30 min→加热回流水提3 h,在此工艺条件下,250 g药材粉,可得干浸膏48.85 g,荷叶碱干重得率0.1697 mg/g,为总体平均得率的1.67倍。影响多糖得率的试验因素主次序为:煎煮时间>药材粉碎>浸泡时间。影响荷叶碱得率的试验因素主次序为:药材粉碎>煎煮时间>浸泡时间。

关键词: 多花黄精复方, 多糖, 荷叶碱, 水提, 工艺, 研究

Abstract: In order to study the extraction technology and method for rhizoma polygonati polysaccharide and nuciferine from Compound Polygonatum Cyrtonema,orthogonal experiments were conducted on 3 factors and 3 levels with water extraction for the effects of the rhizoma polygonati polysaccharide and nuciferine content.The result demonstrated that the best extraction method for rhizoma polygonati polysaccharide was dried herbs pieces over 50,soak 30 min,water heating reflux 2 h.Under the condition of the process,available dry extract could be 54.81g and the yield of rhizoma polygonati polysaccharide could be 64.36%,1.15 times than the average value when 250 g dried herbs were extracted.The best extraction method for nuciferine was dried herbs pieces over100,soak 30 min,water heating reflux 3 h.Under the condition of the process,available dry extract could be 48.85 g and the yield of nuciferine could be 0.1697 mg/g,1.67 times than the average value when 250 g dried herbs were extracted.Primary and secondary order of the main factors influencing the yield of rhizoma polygonati polysaccharide was :decoction time> herbs pieces > soaking time,while herbs pieces > decoction >soaking time for nuciferine.

Key words: Compound Polygonatum Cyrtonema, Rhizoma Polygonati Polysaccharide, nuciferine, water extraction, ethanol extraction, technology