关键词: 金花茶叶多糖, DEAE-纤维素分离, 凝胶色谱纯化, GPC检测, 多糖分析

Abstract: Crude polysaccharides (CCTLP) were extracted from dry leaves of Camellia chrysantha (Hu)Tuyama using water.The four parts including CCTLP-0,CCTLP-0.15,CCTLP-0.3 and CCTLP-0.45 were obtained from CCTLP through sequentially gradient elution by water,0.15 mol/L NaCl,0.3 mol/L NaCl and 0.45 mol/L NaCl as elution reagent by DEAE-cellulose column after removing pigments of CCTLP by D101 macroporous resin.CCTLP-0 and CCTLP-0.15 were then deproteinized by Sevage method,running water dialysis and removing residual NaCl by Sephadex G-15 chromatography for further purification.The five fractions including CCTLP-0-1,CCTLP-0.15-1,CCTLP-0.15-2,CCTLP-0.15-3 and CCTLP-0.15-0.4 were obtained by purification from CCTLP-0 and CCTLP-0.15 using agarose Sepharose 6FF column chromatography.The results showed that they were homogeneous polysaccharide through GPC detection in molecular weight and molecular weight distribution and in analysis of GPC peak type detected.Both CCTLP-0 and CCTLP-0.15 were mainly composted of rhamnose,fructose and galactose through HPLC analysis after they were acid hydrolysis,which molar mass ratios were similar to 1.00:1.066:0.384 and 1.00:1.186:0.544,respectively.

Key words: polysachaide from Camellia chrysantha (Hu) Tuyama leaves, DEAE-cellulose column separation, gel permeation chromatography purification, GPC detection, polysaccharide analysis