天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (10): 1759-1765.doi: 10.16333/j.1001-6880.2017.10.021

• 研究论文 • 上一篇    下一篇

不同炮制时间对何首乌成分及含量的影响

周忆梦,赵梦杰,龚小红,党珏,袁岸,李燕,李芸霞*   

  1. 成都中医药大学药学院 教育部中药材标准化重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都 611137
  • 出版日期:2017-10-27 发布日期:2017-10-27
  • 基金资助:

    中国科技部和澳门科学技术发展基金(007/2014/AM J);开放基金的中药质量研究国家重点实验室(009);中国国家自然科学基金(81373943,81373583);四川青年科学技术基金会(2013JQ0018);四川省科技厅青年科技创新研究团队项目(2015TD0028,2016TD0006,2017TD0001)

The Influence of Different Processing Time on Composition and Content of Polygoni Multiflori

ZHOU Yi-meng, ZHAO Meng-jie, GONG Xiao-hong, DANG Jue, YUAN An, LI Yan, LI Yun-xia*   

  1. Pharmacy College, Chengdu University of Traditional Chinese Medicine; The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine; State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources Co-founded by Sichuan Province and MOST, Chengdu 611137,China  
  • Online:2017-10-27 Published:2017-10-27

摘要: 研究何首乌最佳炮制时间及其成分的含量变化特征。采用HPLC法测定6个不同炮制时间的何首乌对比市售制首乌中各成分的含量,并采用指纹图谱相似性分析、聚类分析以及PLS回归分析对测定结果进行特征分析。结果表明相似度均在0.9~1.0之间,各个样本之间有较好的相关性。聚类分析方法将6个不同炮制时间的何首乌按成分变化特征聚为4类。PLS回归分析法得到二苯乙烯苷和白藜芦醇为不同炮制时间的成分含量变化的主要差异成分。4 h为何首乌最佳炮制时间,为何首乌的质量控制以及深入研究提供参考依据。

关键词: 何首乌, 炮制时间, 相似性分析, 聚类分析, PLS回归分析

Abstract: To study the content variation of chemical composition and best processing time for Polygoni Multiflori Radix.The content of Polygoni Multiflori Radix component with 6 different processing times were determined by HPLC and contrasted with market sale system.Fingerprint similarity analysis,cluster analysis and partial least squares regression analysis were adopted to analyze experimental data.The results demonstrated that the similarity was between 0.9 and 1.0,and there was a good correlation between the samples.Based on the composition of basic characteristics,six Polygoni Multiflori Radix samples were divided into 4 classifications by cluster analysis.PLS regression analysis showed that the content of stilbene glucoside and resveratrol had major differences at different processing times.4h was the best processing time.This study provided reference for quality control and further study of Polygoni Multiflori.

Key words: Polygon multiflora, processing time, similarity analysis, cluster analysis, partial least squares analysis

中图分类号: 

R932