天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (2): 286-293.doi: 10.16333/j.1001-6880.2018.2.019

• 开发研究 • 上一篇    下一篇

乌贼皮胶原蛋白特性与抗氧化能力研究

宋正规,雷静,薛张芝,张洪超,李和生*,王鸿飞,付晶晶   


  1. 宁波大学海洋学院,宁波 315211
  • 出版日期:2018-02-28 发布日期:2018-02-28
  • 基金资助:

    浙江省科技厅重大科技专项(2009C03017-3)

 Characteristics and antioxidant ability of squid skin collagen

SONG Zheng-gui, LEI Jing, XUE Zhang-zhi, ZHANG Hong-chao, LI He-sheng*, WANG Hong-fei, FU Jing-jing   

  1. Ocean College of Ningbo University,NingBo 315211,China
  • Online:2018-02-28 Published:2018-02-28

摘要: 为提高乌贼综合利用率,采用乌贼皮为主要原料,对胃蛋白酶和酒石酸复合提取胶原蛋白的特性与抗氧化性进行综合研究。研究表明:所提取的乌贼皮胶原蛋白溶解性受pH和NaCl浓度影响显著,pH为5时溶解度最小,降低46%;NaCl浓度由0 mol/L增至1.2 mol/L,溶解度逐渐降低80%。黏度受样品浓度、pH、离子强度、贮存时间影响明显,随着样品浓度的增高,黏度逐渐增大;同一浓度,在pH3处黏度取得最大值;随着NaCl浓度的增高,黏度逐渐降低;贮存24 h~48 h,黏度保持最大。抗氧化能力随胶原样品浓度的增加呈上升趋势,DPPH清除率在5 mg/mL时达到最大值49.66%;样品浓度大于10 mg/mL后,还原能力明显增加;胶原样品浓度10 mg/mL时·OH清除率达到最大为95.66%。实验为乌贼皮胶原蛋白的开发利用提供了理论基础。

关键词: 乌贼皮胶原蛋白, 溶解度, 黏度, 抗氧化性

Abstract: In order to improve the comprehensive utilization rate of squid,squid-skin were used as the main raw materials to extract collagen,then the characteristics of collagen extracted from tartaric acid were comprehensively evaluated.The results showed that the collagen had a high solubility,There were a minimum solubility of collagen at the pH of 5,decreased by 46%.When the concentration of NaCl was greater than 0 mol/L,the solubility of collagen decreased as the salt concentration increased.And while the concentration of NaCl increased to 1.2 mol/L,the solubility of collagen decreased by 80%.The collagen concentration,pH,ionic strength and storage time have an effect on viscosity.The viscosity gradually increased with the increased of collagen concentration.The viscosity peaked at pH3.With the increase of NaCl concentration,the viscosity decreases gradually.With the prolonging of the storage time,the viscosity presented a trend of decrease after the first increase,and the viscosity of 24-48 h was the largest.The antioxidant capacity increased with the increase of collagen concentration.Increased collagen concentration,the eliminating ratio of increased gradually, which is 5mg/mL when the maximum file size is reached, 49.66%.Within the range of 2 mg/mL-10 mg/mL,little change in reducing capacity,After more than 10 mg/mL,itsignificantly increased, but still relatively low.With the increase of collagen concentration,the eliminating ratio of hydroxyl radical increased,At 10 mg/mL,the removal rate reached a maximum of 95.66%.This paper supplied the theoretical basis for making full use of the squid-skin collagen.

Key words: protein of collagen from cuttlefish skin, collagen;solubility, viscosity, antioxidant capacity

中图分类号: 

TS254.9 Q58