关键词: 姜黄挥发油, SCl-1细胞, 细胞凋亡, 凋亡相关蛋白

Abstract: To study the effect of turmeric volatile oil (TVO) on the proliferation,migration and invasion of human skin squamous cell carcinoma cell line SCL-1 in vitro,and to explore the mechanism of its apoptosis.The effects of different concentrations and time (24,48,72 h) on the proliferation of SCL-1 cells were detected by MTT;The effects of TVO on migration and invasion of skin squamous cell carcinoma SCL-1 cells were examined by Wound scratch assay and Transwell;Flow cytometry analysis and microscope to determine the apoptosis rate of SCL-1 cells induced by TVO;Western Blot detects Survivin and Caspase-3 protein expression.TVO has inhibitory on the proliferation,migration and invasion of SCL-1 cells (P<0.05) in a time-dose dependent relationship.Flow cytometry analysis showed that TVO can induce apoptosis of SCL-1 cells (P<0.05);The flower-cell analysis and microscopic observation showed that TVO can induce the apoptosis of SCL-1,which was directly related to its concentration;Western blot test results showed that TVO can significantly increase the expression of apoptosis related Caspase-3 (P<0.05),lower the expression of Survivin protein (P<0.05).TVO can significantly inhibit human skin squamous carcinoma SCL-1 cell apoptosis induced by in vitro proliferation,migration,invasion ability and its possible mechanism is by regulating apoptosis related proteins expression of survivin,caspase-3 protein.

Key words: turmeric volatile oil, SCl-1 cell, apoptosis, apoptosis related protein