关键词: 紫苏, 花青素, 提取, 纯化, 分析

Abstract: With perilla as raw material,the anthocyanins in the leaves of different perilla varieties were studied.Perilla leaves were harvested at mature stage and analysis of anthocyanin content in the leaves of perilla varieties.Conventional extraction methods of anthocyanin from plant material are nonselective and yield pigment solutions with large amounts of byproducts such as sugars,organic acids and proteins.Some of these impurities may accelerate anthocyanin degradation.Different extracting media were used and the mixture of ethanol and acidified(50%,v/v)water resulted in maximum anthocyanin content(4.7%).In order to obtain anthocyanins in a purified form,adsorption was carried out with different adsorbents.Among these,nonionic acrylic ester adsorbent,namely Amberlite XAD-7HP showed the highest adsorption capacity and desorption ratio.The main components of anthocyanin were characterized by LCMS.The main components were malonylshisonin.

Key words: Perilla, anthocyanins, extraction, purification, analysis