天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (7): 1188-1194.doi: 10.16333/j.1001-6880.2018.7.016

• 研究简报 • 上一篇    下一篇

RP-HPLC法测定不同产地及不同部位马蓝中六种活性成分的含量

肖春霞1,2，杨万霞2，涂江涛2，苑春茂2，黄烈军2，胡永2，段玉书1,2，顾玮2*，郝小江2,3*   

  1. 1贵州大学药学院，贵阳550025；2省部共建药用植物功效与利用国家重点实验室 贵州省中国科学院天然产物化学重点实验室，贵阳550000；3中国科学院昆明植物研究所 植物化学与西部植物资源持续利用国家重点实验室，昆明 650201
  • 出版日期:2018-08-08 发布日期:2018-08-08
  • 基金资助:

    贵州省自然科学基金（黔科合J字［2015］2105号）；国家自然科学基金（31600280）

     

Determination Analysis of Six Bioactive Constituents Different Parts in Different Habitats of Baphicacanthus cusia (Nees) Bremek by RP-HPLC

XIAO Chun-xia1,2,YANG Wan-xia2,TU Jiang-tao2,YUAN Chun-mao2,HUANG Lie-jun2,HU Yong2,DUAN Yu-shu1,2,GU Wei2*,HAO Xiao-jiang2,3*   

  1. 1College of pharmacy,Guizhou University,Guiyang 550025,China; 2State Key Laboratory of Functions and Applications of Medicinal Plants,The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550000,China; 3State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China
  • Online:2018-08-08 Published:2018-08-08

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摘要： 建立了RP-HPLC法测定马蓝中色胺酮、2-苯并噁唑酮、靛玉红，靛蓝、阿克苷和角胡麻苷六种活性成分含量的方法，并对不同产地及不同部位药材含量进行了分析。采用ZORBAX SB-C18色谱柱（4.6×250 mm，5 μm），以乙腈和水为流动相梯度洗脱，流速为1 mL/min；检测波长280 nm；柱温30 ℃。马蓝中六种化学成分分离良好，各成分含量与峰面积在测定范围内均呈良好的线性关系（r≥ 0.999 1），加样回收率分别为100.25%、99.93%、100.46%、99.94%、99.92%、100.02% ，RSD值分别为 1.46、0.13、0.52、0.09、0.16、0.40。该方法简便快捷、重现性好，可为南板蓝根药材的品质评价和质量控制标准提供科学依据。

关键词: 含量测定, 马蓝, RP-HPLC, 不同产地, 不同部位

Abstract: This research aims to establish a RP-HPLC method for analysis of bioactive constituents such as tryptanthrin,2(3H)-benzoxazolinone,indirubin,indigo,acteoside,martynoside of different parts of Baphicacanthus cusia (Nees) Bremek in different habitats.The chromatographic separation was performed on ZORBAX SB-C18 column（4.6×250 mm,5 μm）with gradient elution consisting of acetonitrile and water at a flow rate 1 mL/min.The UV detection wavelength was 280 nm and the column temperature was set at 30 ℃.The six kinds of compounds in B.cusia separated well,and the linear relation was obtained (r ≥ 0.999 1).The average recoveries were 100.25%,99.93%,100.46%,99.94%,9992% and 100.02%,with RSD 1.46,0.13,0.52,0.09,0.16 and 0.40.The method is simple,quick,and good reproducibility,which can provide the basis for the rational use of the active parts of B.cusia.

Key words: content determination analysis, Baphicacanthus cusia (Nees) Bremek, RP-HPLC, different habitats, different parts

中图分类号: 

R927.2 Q946

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