天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (8): 1387-1391.doi: 10.16333/j.1001-6880.2018.8.017

• 研究简报 • 上一篇    下一篇

银杏酸单体对黄嘌呤氧化酶的体外抑制活性研究

夏宏军1*,褚梦颖1,徐云婷1,吴玲玲1,李巍2   

  1. 1扬州大学医学院药学系;2扬州大学医学院临床医学系,扬州 225001
  • 出版日期:2018-09-04 发布日期:2018-09-04
  • 基金资助:

    江苏省自然科学基金青年基金(BK20130455);扬州大学创新培育基金

Inhibitory Activities of Ginkgolic Acid Monomers from Ginkgo biloba Sarcotesta on Xanthine Oxidase In Vitro

XIA Hong-jun1*,CHU Meng-ying1,XU Yun-ting1,WU Ling-ling1,LI Wei2   

  1. 1Department of Pharmacy,Medical College,Yangzhou University; 2Department of Clinical Medicine,Medical College,Yangzhou University,Yangzhou 225001,China
  • Online:2018-09-04 Published:2018-09-04

摘要: 本研究首次系统评价了银杏酸单体化合物对黄嘌呤氧化酶的体外抑制活性。银杏外种皮的无水乙醇提取物通过开放硅胶柱色谱和半制备型反相高效液相色谱分离,得到五个银杏酸单体化合物。以别嘌呤醇为阳性药物,采用微孔板法评价它们对黄嘌呤氧化酶的抑制作用。每个浓度设4个复孔,使用酶标仪在295 nm波长下测定每孔吸光度,计算平均抑制率;实验重复3次,采用Graphpad prism 5.0 软件计算IC50值。结果表明,银杏酸C13∶0和银杏酸C15∶1对黄嘌呤氧化酶的IC50值分别为31.81±1.87 μmol/L和25.48±0.66 μmol/L(n=3),显示了一定程度的黄嘌呤氧化酶抑制活性。

关键词: 银杏外种皮, 银杏酸单体化合物, 黄嘌呤氧化酶, 抑制活性

Abstract: To study the inhibitory activities of ginkgolic acid monomers from Ginkgo biloba sarcotesta on xanthine oxidase (XOD) in vitro for the first time,the absolute ethanol extract of Ginkgo biloba sarcotesta was separated by open silica gel column chromatography and purified by semi-preparative reversed phase HPLC subsequently,and five ginkgolic acid monomers were obtained.Their inhibitory activities on XOD in vitro were evaluated by microplate-based method,and allopurinol was used as a positive drug.Each concentration had four replicates,and mean inhibition ratios were calculated according to absorbance measured on a microplate reader at 295 nm.The experiments were repeated for three times,and the half inhibitory concentration (IC50) was calculated by using Graphpad prism 5.0 software.Conclusion was made that ginkgolic acid (13∶0) and ginkgolic acid (15∶1) have shown certain inhibitory activities on XOD in vitro,and their IC50 values were 31.81±1.87 μmol/L and 25.48±0.66 μmol/L (n=3),respectively.

Key words: Ginkgo biloba sarcotesta, ginkgolic acid monomers, xanthine oxidase, inhibitory activity

中图分类号: 

R914.4