关键词: 樟树, RAW264.7, 细胞因子, 抗炎作用

Abstract: To observe the anti-inflammatory effect of the residue left after extracting spices from Cinnamomum camphora on LPS-induced RAW 264.7 macrophages.Hexane,dichloromethane(CH₂Cl₂),ethyl acetate(EtOAc),n-butanol (n-BuOH) and water (H₂O) fractions of 95% alcohol aqueous extracts of C.camphora were obtained.Then,the effects of five fractions of different concentrations on RAW264.7 cell viability were determined with the MTT method.NO concentration was measured with Griess method.The enzyme linked immunosorbent assay (ELISA) was used to detect the secretion of PGE₂ and TNF-α.The reverse transeriptase-polymerase chain reaction (RT-PCR) and real time RT-PCR were used to determine the expression of COX-2、iNOS and TNF-α mRNA.Pre-treatment with EtOAc fraction of C.camphor showed no cytotoxicity can significantly inhibited the LPS-stimulated secretion of NO,PGE₂,and TNF-α into the culture medium,as well as the mRNA levels of inducible nitric oxide synthase (iNOS),cyclooxygenase-2 (COX-2),and TNF-α.Overall,our data suggest that EtOAc fraction of C.camphor possess significant anti-inflammatory properties by down-regulation of COX-2、iNOS and TNF-α expression from transcriptional levels.EtOAc fraction of C.camphor may exert anti-inflammatory effect on RAW264.7 cells stimulated by LPS．

Key words: Cinnamomum camphora, RAW 264.7, cytokine, anti-inflammatory effect