天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (9): 1543-1547.doi: 10.16333/j.1001-6880.2018.9.012

• 研究简报 • 上一篇    下一篇

基于主成分分析与聚类分析比较研究南沙参挥发性成分

周坤1,江汉美2*,许家琦2,郭彧2   

  1. 1华中科技大学同济医学院附属精神卫生中心药学部,武汉 430022;2湖北中医药大学制药工程教研室,武汉 430065
  • 出版日期:2018-10-08 发布日期:2018-10-10

Comparative Study on Volatile Constituents of  Radix Adenophorae Based on Principal Component Analysis and Cluster Analysis

ZHOU Kun1,JIANG Han-mei2*,XU Jia-qi2,GUO Yu2   

  1. 1Affiliated Mental Health Center Department of Pharmacy,tongji Medicial College of Huazhong University of Science & Technology,Wuhan 430022,China ; 2Hubei University of Chinese Medicine Department of Pharmaceutical Engineering & Teaching,Wuhan 430065,
  • Online:2018-10-08 Published:2018-10-10

摘要: 采用固相微萃取(SPME)对南沙参挥发性成分进行萃取,结合气相色谱-质谱联用 (GC-MS)对其进行分析;以峰面积归一化法计算各组分的相对百分含量,并采用SPSS 18.0软件对结果进行主成分分析和聚类分析。结果表明,从不同产地南沙参中共分离鉴定出41种化学成分,其主要成分为烷酸、烯酸、醛类和醇类化合物;其中异缬草醛、己醛、α-蒎烯、羊脂醛为5个不同产地南沙参的共有成分,江苏、湖北产的南沙参中含有丰富的萜类化合物;聚类分析结果可将不同产地南沙参划分为5类。本实验建立了南沙参挥发性成分的快速分析方法,获取了不同产地南沙参挥发性成分的特征分布和产地信息鉴别模式。主成分分析法和聚类分析法能有效区分不同产地的南沙参,研究结果可为南沙参药材品种、鉴别比较提供参考依据。

关键词: 南沙参, 固相微萃取, 气相色谱-质谱, 挥发性成分, 聚类分析, 主成分

Abstract: The volatile constituents were extracted from Radix Adenophorae by solid phase micro extraction,and were qualitified by gas-chromatography mass-spectrometry,and content of each component of volatile constituents were calculated by peak area normalization method.by SPSS 18.0 software,principal component analysis and cluster analysis were used to statistical analysis.41 kinds of constituents were determind in Radix Adenophorae which come from different habitats,the main components are alkylic acid,olefine acid,aldehydes and alcohols; Radix Adenophorae which come from different habitats were contains isovaleraldehyde,hexanal,α-pinene and suet aldehyde; Radix Adenophorae which come from Jiangsu and Hubei were contain more terpenoids. Radix Adenophorae which come from different habitats can be divided into five categories by cluster analysis.the study established a rapid analysis method of the volatile components of Radix Adenophorae and contributed the patterns of characteristics distribution and origin information of volatile components from Radix Adenophorae,the method is simple and fast.principal component analysis and cluster analysis method can effectively distinguish  Radix Adenophorae which come from different areas.the results can provide evaluation method for the species and quality comparison of Radix Adenophorae.

Key words: Radix Adenophorae, solid phase micro extraction, gas-chromatography-mass spectrometry, volatile components, cluster analysis, principal component analysis

中图分类号: 

R284.1 Q946