天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (10): 1758-1763.doi: 10.16333/j.1001-6880.2018.10.017

• 研究简报 • 上一篇    下一篇

聚酰胺分离低极性萜类成分和脂溶性色素的应用研究

韩曼飞,张刘强*,李医明*   

  1. 上海中医药大学中药学院,上海 201210
  • 出版日期:2018-10-31 发布日期:2018-11-01
  • 基金资助:

    国家自然科学基金(81673570);上海市优秀学术带头人计划(16XD1403500);上海市“中药药效物质”E-研究院项目

Application of Separation Lower Polar Terpenoids and Lipophilic Pigment by Polyamide

HAN Man-fei,ZHANG Liu-qiang*,LI Yi-ming*   

  1. College of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201210,China
  • Online:2018-10-31 Published:2018-11-01

摘要: 以穿心莲、冬凌草、银杏叶、枇杷叶4种含有不同萜类化合物的中草药为试材,在乙醇-水体系下,运用聚酰胺柱色谱对茎叶类中药中低极性萜类成分和脂溶性色素进行分离研究。实验结果表明,聚酰胺柱层析法可安全有效脱去脂溶性色素,而达到富集低极性萜类化合物的效果。这可解决硅胶等柱色谱进行此类成分分离所带来的安全性和分离效果差的问题,同时为萜类成分的产业化富集提供了新的方法。

关键词: 聚酰胺, 醇水体系, 萜类化合物, 脂溶性色素, 分离纯化

Abstract: To isolate lower polar terpenoids and lipophilic components from aerial part of Andrographis paniculata and Rabdosia rubescens,from leaves of Ginkgo biloba and Eriobotrya japonica by polyamide column chromatography,using the ethanol-water system.The result showed that lipophilic pigment could safely and effectively be taken off,and the lower polar terpenoids could be enriched through polyamide column chromatography.The above work will help to solve the problem of resolution and safety induced by other column chromatography,and to provide a new method for the industrialized enrichment of terpenoids.  

Key words: polyamide, ethanol-water system, terenoids, lipophilic pigment, purification

中图分类号: 

R932