关键词: 青橄榄浸膏, 总黄酮, 总多酚, 超声波, 得率, 抗氧化

Abstract: In order to optimize extraction of extractum from Canarium album and investigate its antioxidant activity.Canarium album from Maoming was used as raw material to prepare extractum from fruit pulp by ultrasonic assisted ethanol.Taking the total flavonoids and total polyphenols yield as evaluation indexes,the effects of various factors on the extraction of olive extractum were studied.The antioxidant activity of olive extractum was evaluated by the method of ortho three phenol autoxidation,crystal violet and DPPH scavenging capacity.The results showed that the optimum extraction process of extractum was as follows∶ethanol volume fraction 70%,solid-liquid ratio at 1∶18 (g∶mL),ultrasonic extraction temperature 50 ℃,time 6 min (ultrasonic extraction stage),pure organic solvent extraction temperature 60 ℃,time 45 min (organic solvent extraction stage).Under this condition,total flavonoids yield was 1.76%,and total polyphenols yield was 15.53%.The inhibition rate of the superoxide anion radical of the final product under 0.3 mg/mL concentration was 22.74%,equivalent to 23.47% of the same mass concentration of ascorbic acid,and the clearance rate of hydroxyl radical at 0.02 mg/mL concentration was 67.32%,equivalent to 112.58% of the scavenging effect of the same mass concentration of anti blood acid;in 0.2 mmol/mL In the DPPH solution system,the clearance rate of 0.15 mg/mL extract to DPPH was 95.40%,equivalent to 140.83% of the scavenging effect of the same mass concentration of ascorbic acid.Therefore,in general,the extractum has a good antioxidant activity,although the inhibition rate of superoxide anion radical was weaker than that of ascorbic acid,the clearance rate of hydroxyl radical and DPPH was better than that of ascorbic acid.

Key words: Canarium album extractum, total flavonoids, total polyphenols, ultrasonic wave, yield, antioxidant activity