附子不同组分抗急性心力衰竭大鼠的实验研究

doi:10.16333/j.1001-6880.2019.3.006

天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (3): 408-414.doi: 10.16333/j.1001-6880.2019.3.006

附子不同组分抗急性心力衰竭大鼠的实验研究

陈海媚1,2，陈秋伶1，李梦婷1,2，谢晓芳1,2*，彭成1,2*

1成都中医药大学药学院；2中药资源系统研究与开发利用省部共建国家重点实验室培育基地，成都 611137

出版日期:2019-04-02 发布日期:2019-04-02
基金资助:
国家自然科学基金重点项目（81630101）；国家发改委标准化项目（ZYBZH-C-SC-51）；四川省中管局项目（2016ZY008）；国家科技部科技基础工作专项（2015FY111500-140）；四川省中央引导地方科技发展专项（2017JZYD0001）

Study on the effects of different components of Radix Aconiti Lateralis on rats with acute heart failure

CHEN Hai-mei1,2,CHEN Qiu-ling1,LI Meng-ting1,2,XIE Xiao-fang1,2*,PENG Cheng1,2*

1Pharmacy College,Chengdu University of Traditional Chinese Medicine; 2State Key Laboratory Breeding Base of Systematic Research,Development and
Utilization of Chinese Medicine Resources Co-founded by Sichuan Province and MOST,Chengdu 611137,China

Online:2019-04-02 Published:2019-04-02

摘要/Abstract

摘要： 从血流动力学和神经体液因子两方面探讨附子不同组分对大鼠急性心力衰竭模型的治疗作用。采用盐酸普罗帕酮复制急性心力衰竭模型，记录造模前后以及给药后5、10、20、30、60 min大鼠心脏频率（HR）、左心室最大上升速率（+dp/dt_max）和左心室最大下降速率（-dp/dt_max）值的变化，并测定血清中Ang-I、Ang-Ⅱ、TNF-α和ANP含量。结果显示，正常大鼠造模后，其HR和+dp/dt_max明显降低，-dp/dt_max明显升高，且Ang-Ⅰ、Ang-II、TNF-α、ANP均有升高趋势。与模型组比较，总生物碱组和水溶性生物碱组可不同程度的升高模型大鼠各时间点的HR、+dp/dt_max，降低-dp/dt_max；且总生物碱组、水溶性生物碱组、脂溶性生物碱组和多糖组均有一定降低模型大鼠血清中Ang-Ⅰ、Ang-II、TNF-α、ANP水平含量的趋势。结果表明总生物碱对改善血流动力学作用最强，其次是水溶性生物碱，且两者均能显著降低血清中与心力衰竭有关的神经体液因子，有效治疗急性心力衰竭，由此可见，附子水溶性生物碱以及总生物碱是其发挥强心作用的主要物质基础。

关键词: 附子, 不同组分, 急性心力衰竭

Abstract: To study the effects of different components of Radix Aconiti Lateralison rats with acute heart failure (AHF) in hemodynamics and neurohumoral factors.Use propafenone hydrochloride to copy the model rats of AHF.Then,observe and record the change of HR,+dp/dt_max and-dp/dt_max after administration 5,10,20,30,60 min,and measure the concentration of Ang-Ⅰ,Ang-II,TNF-α,ANP by blood sampling after 60 min by ELISA.After modeling with propafenone hydrochloride,HR,+dp/dt_max were significantly decreased and-dp/dt_max was significantly increased,and Ang-Ⅰ,Ang-II,TNF-α,ANP were trend to rise.Compared with the model group,total alkaloids and water-soluble alkaloids can increase the HR,+dp/dt_max and decrease the-dp/dt_max in varying degrees at all points of time;total alkaloids,water-soluble alkaloids,fat-soluble alkaloids and polysaccharides had the tendency to reduce the concentration of Ang-Ⅰ,Ang-II,TNF-α,ANP,which were increased by modeling.In conclusion,the total alkaloids had the most significant effect of improving hemodynamics,followed by the water-soluble alkaloids,both of which could affect the neurohumoral factors associated with heart failure.Thus,it can be seen that the water-soluble alkaloids and total alkaloids are the main material basis in playing the role of cardiac.

Key words: Radix Aconiti Lateralis, different components, acute heart failure

中图分类号:

R285.5 R965

陈海媚1,2，陈秋伶1，李梦婷1,2，谢晓芳1,2*，彭成1,2*. 附子不同组分抗急性心力衰竭大鼠的实验研究[J]. 天然产物研究与开发, 2019, 31(3): 408-414.

CHEN Hai-mei1,2,CHEN Qiu-ling1,LI Meng-ting1,2,XIE Xiao-fang1,2*,PENG Cheng1,2*. Study on the effects of different components of Radix Aconiti Lateralis on rats with acute heart failure[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2019, 31(3): 408-414.

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附子不同组分抗急性心力衰竭大鼠的实验研究

Study on the effects of different components of Radix Aconiti Lateralis on rats with acute heart failure

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