关键词: 芳姜黄酮, 皮肤鳞状细胞癌A431细胞, 凋亡, Notch1, Hes1, PTEN

Abstract: In order to study the effects of Ar-Turmerone on proliferation,migration,invasion and apoptosis of human squamous cell carcinoma A431 cells,CCK-8 method was used to detect the inhibition rate of Ar-Turmerone on A431 cells.Cell morphology was observed by Giemsa staining,cell migration ability was analyzed by scratch assay;cell invasion ability was studied by Transwell chamber experiment,apoptosis rate was detected by flow cytometry.The expression of mRNA was detected by real-time PCR,and the protein was detected by western blotting.The expression of Notch1,Hes1 and PTEN was blocked by siRNA,the expression of corresponding downstream mRNA and protein was detected,and the apoptosis rate was detected by flow cytometry.It was found that the action of Ar-Turmerone on A431 cells can inhibit cell proliferation,affect cell morphology,and inhibit cell migration and invasion,and promote cell apoptosis.After treatment,Notch1,Hes1,PTEN mRNA and protein expression were elevated.Silencing Notch1,Hes1 mRNA and protein expression were lower than the simple administration group,while silencing Hes1,PTEN mRNA and protein expression was also lower than the simple administration group;after silencing PTEN,compared with the simple drug-administered group,the death rate of the cell is reduced.In conclusion,it can be proved that the Ar-Turmerone can inhibit the proliferation of human squamous cell carcinoma A431 cells and promote its apoptosis;it can inhibit migration and invasion in vitro;the mechanism of Ar-Turmerone promoting apoptosis is through Notch1/Hes1 /PTEN pathway is implemented.

Key words: Ar-Turmerone, hauman squamous cell carcinoma A431 cells, apoptosis, Notch1, Hes1, PTEN