天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (6): 975-980.doi: 10.16333/j.1001-6880.2019.6.008

• 研究论文 • 上一篇    下一篇

蒲公英植酸对沙门氏菌抑制作用及其抑菌机理研究

高飞雄,梁引库*,李云祥   

  1. 陕西理工大学生物科学与工程学院,汉中 723000
  • 出版日期:2019-06-26 发布日期:2019-06-26
  • 基金资助:

    陕西理工大学人才启动项目(SLGQD1814);陕西省教育厅重点实验室项目(18JS018)

Antibacterial effect and mechanism of dandelion phytic acid on Salmonella

GAO Fei-xiong,LIANG Yin-ku*,LI Yun-xiang   

  1. School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong,723000,China
  • Online:2019-06-26 Published:2019-06-26

摘要: 为探究蒲公英植酸对沙门氏菌的抑制作用及其抑菌机理。本文利用沉淀法和离子交换法提取蒲公英植酸,滤纸片法分析蒲公英植酸对沙门氏菌(Salmonella)的抑菌作用,倍比稀释法研究蒲公英植酸的最低抑菌浓度。通过分析沙门氏菌的细胞通透性和生长动力学,结合扫描电镜和荧光显微镜研究了蒲公英植酸对沙门氏菌的抑菌机理,表明蒲公英植酸对沙门氏菌具有很好的抑菌能力,其最小抑菌浓度为0.2 mg/mL。而且植酸对沙门氏菌的抑制作用是通过破坏细胞膜达到抑菌的效果,并且植酸浓度越高,抑菌效果越显著。这表明蒲公英植酸可以有效地抑制沙门氏菌生长,其主要是通过破坏菌体细胞膜完整性,增加细胞薄膜的通透性,使细胞内容物外溢达到抑制细菌生长的目的。

关键词: 蒲公英, 植酸, 沙门氏菌, 抑菌作用, 抑菌机制

Abstract: To explore the antibacterial effect of dandelion phytic acid on Salmonella and its antibacterial mechanism.In this paper,the methods of precipitation and ion exchange were used to extract phytic acid from dandelion.The antibacterial effect of dandelion phytic acid on Salmonella was analyzed by filter paper method and the minimum inhibitory concentration of dandelion phytic acid was studied by dilution method.And the bacteriostatic mechanism of dandelion phytic acid against Salmonella was explored by analyzing the cell permeability and growth kinetics of Salmonella,combined with scanning electron microscopy and fluorescence microscopy.It indicated that dandelion phytic acid had good antibacterial ability against Salmonella and its minimum inhibitory concentration was 0.2 mg/mL.Moreover,the inhibitory effect of phytic acid on Salmonella was achieved by destroying cell membrane,and the higher the phytic acid concentration,the more significant the bacteriostatic effect.It is indicated that dandelion phytic acid can effectively inhibit the growth of Salmonella,mainly by destroying the cell membrane integrity of the cells,increasing the permeability of the cell membrane,and allowing the cell contents to overflow to inhibit the growth of bacteria.

Key words: dandelion, phytic acid, Salmonella, bacteriostatic characteristics, antibacterial mechanism

中图分类号: 

R285