关键词: 羊角天麻, 化学成分, &beta, -羟高铁血红素形成抑制活性

Abstract: To study the inhibitory active constituents from Dobinea delavayi against β-hematin formation.The compounds were isolated by silica gel and sephadex LH-20 colunm chromatography,and their structures were identified by the analyses of their physicochemical properties and NMR data.Twelve compounds were isolated and identified as 3,4,2′,4′-tetrahydroxydihydrochalcone (1),butin (2),3,4,2′,4′,α-pentahydroxychalcone (3),aureusidin (4),mangiferin (5),gallic acid (6),24-metbylenecycloartanol (7),6β-hydroxystigmast-4-en-3-one (8),β-sitosterol (9),daucosterol (10),methyl hexadecanoate (11),and glyceroylmonopalmitate (12).Compounds 1-4 and 6-8 were isolated from the Dobinea for the first time.According to the bioassay experiment,compounds 1-6 showed β-hematin formation inhibition activities in different degrees,and the flavonoids compounds 2-4 revealed significant β-hematin formation inhibition activities with IC₅₀ of 120.3,61.5 and 56.0 μg/mL,respectively

Key words: Dobinea delavayi, chemical constituents, β-hematin formation inhibitory activity