天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (增刊1): 30-34.doi: 10.16333/j.1001-6880.2019.S.006

• 研究论文 • 上一篇    下一篇

基于花色苷苷元组分分析的黑枸杞原产地溯源方法研究

燕宇真,王慧春,曾阳*, 王自超*   

  1. 青海师范大学生命科学学院,西宁 810099
  • 出版日期:2019-08-06 发布日期:2019-08-06
  • 基金资助:

    国家自然科学基金(81460652);青海省基础研究项目(2017-ZJ-774);青海师范大学科研基金

Establishing a method to trace geographical origins of Lycium ruthenicum Murray based on the analysis of its anthocyanin aglycone compositions

YAN Yu-zhen, WANG Hui-chun, ZENG Yang*, WANG Zi-chao*   

  1. School of Life science, Qinghai Normal University, Xining 810099, China
  • Online:2019-08-06 Published:2019-08-06

摘要: 采用高效液相色谱法测定了不同产地黑枸杞样品中的花色苷苷元的组分。发现黑枸杞中花色苷苷元种类丰富,且矮牵牛的含量最高,为5 776.42~14 858.52 mg/kg,说明黑枸杞具有很好的营养价值和生物活性。另外,黑枸杞中的花色苷苷元含量因黑枸杞的产地不同而呈现出较大的差异,因此可用于多元统计分析来进行黑枸杞原产地溯源。分析结果显示,黑枸杞花色苷苷元祖分分析结合主成分分析和聚类分析,可用于黑枸杞原产地的溯源,识别能力和预判率均达到了100.0%。

关键词: 黑枸杞, 花色苷苷元, 多元统计分析, 原产地溯源

Abstract: High performance liquid chromatography was used to measure anthocyanin aglycone compositions in Lycium ruthenicum Murray from different regions. It was observed that Lycium ruthenicum Murray has most all the anthocyanidin in nature, with petunidin having the highest level about 5 776.42-14 858.52 mg/kg, indicating Lycium ruthenicum Murray has good nutritional value and biological activity. In addition, the contents of anthocyanin aglycone varied greatly due to different geographical origins, suggesting that anthocyanin aglycone compositions can be used to distinguish Lycium ruthenicum Murray from different geographical origins using multivariate statistical analysis which showed a recognition ability of 100.0% and a prediction ability of 100.0%. The results showed that analysis of anthocyanin aglycone compositions combined with principal component and linear discriminant analysis could be a good method to trace the geographical origin of Lycium ruthenicum Murray.

Key words: Lycium ruthenicum Murray, anthocyanin aglycones, multivariate statistical analysis, tracing geographical origins

中图分类号: 

Q946.91