关键词: 明党参内生菌, 呋喃香豆素, 生物转化, 高效液相色谱

Abstract: To explore endophytes from Changium smyrnioides Wolff have biotransformation effects on its three furanocoumarins components,the endophytic strains from Changium smyrnioides Wolff were separated by plate culture method.After fermentation of Changium smyrnioides Wolff raw powder culture medium of endophytes from Changium smyrnioides Wolff,the content of three furanocoumarin components of imperatorin,isoimperatorin and psoralen was determined by HPLC. The samples were separated on a Promosil C₁₈-BIO column (4.6 mm×250 mm,5 μm) with acetonitrile-water (80∶20) as the mobile phase，the flow rate was 1.0 mL/min,the temperature of column was 30 ℃,and the detection wavelength was 203 nm. Strains NMY1,PMG3,NMG10 and NMJ10 can increase the content of three furocoumarin components,among which the strains NMJ10 and NMY1 have the best promoting effect. The endophytes NMY1 and NMJ10 had the highest total amount of furocoumarins by fermenting the Changium smyrnioides Wolff culture liquid at 24 h,and the strains PMG3 and NMG10 had the highest total content of the three furocoumarin components at 48 h of fermentation. The increasing proportions of strains NMJ10,NMY1 and NMG10 on the total content of three furocoumarins were 17.47%,17.26% and 16.16%,respectively. The strains have a significant improvement on the content of imperatorin in the whole fermentation process,and it is more conducive to the conversion synthesis of imperatorin components. It is studied that the endophytes NMY1,PMG3,NMG10 and NMJ10 from Changium smyrnioides Wolff have biotransformation effects on the content of three furanocoumarins components.

Key words: endophytes of Changium smyrnioides Wolff, furanocoumarin, biotransformation, HPLC