关键词: 桦褐孔菌, 三萜, 紫外分光光度法, 响应曲面, 大孔吸附树脂

Abstract: In order to optimize extraction technology of Inonotus obliquus triterpenoids,and investigate its purification technology by macroporous resin.This experiment with the content of total saponins as index,extract solvent,solid-liquid ratio,extraction temperature,extraction time and times were selected as factors,the single-factor investigation and response surface method were used to screen the extraction process of Inonotus obliquus triterpenoids.With sample concentration,sample volume,flow rate on sample PH,concentration and volume of eluent were selected was used to investigate purification technology.The single-factor investigation was used to AB-8 macroporous resin purification process.The MTT test validates its anti-gastric activity.The results showed that ethanol was used as the extraction solvent,90 Hz,ultrasonic for 30 min,the solvent ratio was 1∶15 and extraction was 82 ℃ for 2 h.The optimal purification process for Inonotus obliquus triterpenes∶1 mg/mL for sample loading,2 BV for sample loading,optimal pH=5 for sample loading,2 mL/min for optimum flow rate,95% ethanol for eluent,pH=5.using purified Inonotus obliquus triterpenes can significantly inhibit HeLa cell growth.The extraction and purification process is feasible.And lay the foundation for the development of Inonotus obliquus triterpenoids.

Key words: Inonotus obliquus, triterpene, ultraviolet spectrophotometry, response surface method, macroporous resin