关键词: 高效液相色谱-衍生化, 桑叶, DNJ, Fagomine, 提取, 纯化

Abstract: A high performance liquid chromatography-derivatization method was established to simultaneously detect the contents of 1-deoxynojirimycin (DNJ) and Fagomine in mulberry leaves,and the extraction and purification processes of DNJ and Fagomine in mulberry leaves under the same conditions were studied by resin method.Different extraction solvents,different types of resin,resin dynamic adsorption characteristics,parameters such as concentration of eluant,and the high performance liquid chromatography (HPLC) of DNJ in mulberry leaves and Fagomine derivatization method for testing,mulberry DNJ and Fagomine optimization conditions of extraction and purification process,mulberry leaf meal to add 6 times the amount of 0.1% hydrochloric acid aqueous solution,heating reflux extraction,three times after the extract concentration on LSD001 resin column,then in 0.5 mol/L of ammonia elution and collect the eluent.The content of DNJ and Fagomine reached 5.65% and 1.35% respectively.

Key words: high performance liquid chromatography-derivatization method, mulberry leaves, DNJ, Fagomine, extraction, purification