关键词: 美花圆叶筋骨草, 化学成分, 抗炎活性, PHGDH抑制活性

Abstract: To investigate the chemical constituents and biological activity of Ajuga ovalifolia var. calantha.The 70% acetone extracts from the whole herb of Ajuga ovalifolia var. calantha were separated and purified by column chromatography over silica gel,Sephadex LH-20,ODS and RP-HPLC.Their chemical structures were elucidated on the basis of physicochemical characteristics and spectral analyses.Twelve compounds were isolated and identified as 2-methoxy-4-(2-propenyl)-phenyl-β-D-glucopyranoside (1),oct-1-en-3-yl-β-glucopyranoside (2),isomartynoside (3),20-hydroxyecdysone-20,22-monoacetonid (4),octyl-β-D-Glucopyranoside (5),(2S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D-galactopyra-noside (6),martynoside (7),8-acetylharpagide (8),polypodine B (9),acetovanillone (10),ajugalactone (11),α-(9Z,12′Z,15′Z)-octadecatrienoic acid monoglyceride (12).Compounds 1-6,8 and 12 were isolated from genus Ajuga for the first time,and compounds 1-9,11-12 were isolated from this plant firstly.Lipopolysaccharide(LPS)-stimulated RAW264.7 macrophages were used to assess the anti-inflammatory activity of some compounds from Ajuga ovalifolia var. calantha,and their inhibitory activities to phosphoglycerate dehydrogenase (PHGDH) were tested.The compound 3,4,7,9,11 had no anti-inflammatory activities,and compound 7 showed some inhibitory activity to PHGDH.

Key words: Ajuga ovalifolia var. calantha, chemical constituents, anti-inflammatory activity, PHGDH inhibited activity