楮实子对药物性肝损伤大鼠氧化应激因子的影响

doi:10.16333/j.1001-6880.2019.9.020

天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (9): 1617-1623.doi: 10.16333/j.1001-6880.2019.9.020

所属专题： No.2

楮实子对药物性肝损伤大鼠氧化应激因子的影响

王茜，张一昕*，石铖，郝蕾，韩雪，郭秋红，张晓栋

河北中医学院药学院，河北省高校中药组方制剂应用技术研发中心，石家庄 050200

出版日期:2019-10-09 发布日期:2019-10-23
基金资助:
国家自然基金青年基金(81503268）；河北省高等学校科学技术研究青年拔尖计划(BJ2016038）；中央财政公共卫生专项“2017年中药资源普查项目”(Z135080000022）

Effect of Broussonetiae Fructus on oxidative stress factors in rats with drug-induced liver injury

WANG Xi，ZHANG Yi-xin*，SHI Cheng，HAO Lei，HAN Xue，GUO Qiu-hong，ZHANG Xiao-dong

Hebei College of Traditional Chinese Medicine,Hebei Higher Education Institute Applied Technology Research Center on TCM Formula Preparation,Shijiazhuang 050200,China

Online:2019-10-09 Published:2019-10-23

摘要/Abstract

摘要： 为了探讨楮实子对对乙酰氨基酚（APAP）诱导的药物性肝损伤大鼠的保护作用以及对过氧化物酶体增殖物激活受体γ（PPAR-γ）、过氧化物酶体增殖物激活受体α（PPAR-α）、C-Ros癌基因1（ROS1）的调控作用。实验将50只SD大鼠随机分为正常组、模型组、水飞蓟宾组（44 mg/kg）和楮实子高、低剂量组（4.2、1.05 g生药/kg），每组10只。灌胃给予对乙酰氨基酚（1.2 kg/kg）制备肝损伤模型，给药组造模的同时给予相应药物治疗，连续30天。实验结束，收集血清、肝组织标本进行指标检测。结果显示，楮实子各剂量均能降低药物性肝损伤大鼠血清中谷丙转氨酶（ALT）和谷草转氨酶（AST）活性，降低总胆红素（TBIL）和直接胆红素（TBIL）的含量，升高血清中谷胱甘肽（GSH）含量、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性，降低丙二醛（MDA）含量以及ROS1的表达，上调PPAR-α mRNA的表达，下调PPAR-γ mRNA的表达。以上研究结果表明，楮实子能防治对乙酰氨基酚所致肝损伤，其作用机制可能是通过降低ROS1的表达、调节转录因子PPAR-α和PPAR-γ的基因表达，从而缓解氧化应激损伤来实现的。

关键词: 楮实子；药物性肝损伤；ROS1；PPAR-&alpha, ；PPAR-&gamma,

Abstract: To investigate the protective effect of Broussonetiae Fructus on acetaminophen-induced liver injury in rats and the regulatory effects on peroxisome proliferators activated receptor α (PPAR-α),peroxisome proliferators activated receptor γ (PPAR-γ),and C-Ros oncogene 1 (ROS1).50 SD rats were randomly divided into normal group,model group,silymarin group (44 mg/kg),Broussonetiae Fructus high and low dose groups (4.2，1.05 g crude drug/kg) with 10 rats in each group.The rat model of drug-induced liver injury was induced by acetaminophen (1.2 g/kg) orally once daily for 30 days.At the same time of modeling,normal group and model group were given saline infusions by gastric lavage,while the rest groups were given corresponding liquid infusions for 30 days.At the end of the experiment,the activities of AST,ALT,SOD,CAT and GSH-Px,and the contents of TBIL,DBIL,MDA and GSH in serum were measured.Part of the liver tissue was fixed with 4% paraformaldehyde solution,the pathological morphology of the liver was observed with HE staining,and the gene expression of PPAR-α and PPAR-γ were observed by RT-PCR,and the protein expression of ROS1 (C-Ros oncogene 1) was observed by immunohistochemistry.Our results showed that compared with the normal group,the levels of ALT,AST,TBIL and DBIL in rats of the model group were significantly increased,the content of MDA in serum and the gene expression of PPAR-γ in liver were increased,while the levels of SOD,GSH,GSH-Px and PPAR-α were decreased.Compared with the model group,different doses of Broussonetia Fructus could reduce the levels of ALT,AST,TBIL and DBIL,increase the levels of SOD,GSH,GSH-Px in serum,inhibit the gene expression of PPAR-γ and the protein expression of ROS1,and up-regulate the gene expression of PPAR-α.In conclusion,the results of this study suggest that Broussonetiae Fructus may have protective effect on the hepatic injury induced by acetaminophen in rats,and its mechanism may be related to the inhibition of oxidative stress and regulation the gene expression of PPAR-γ,PPAR-α,and the expression of ROS1 protein.

Key words: drug-induced liver injury, fructus broussonetia, ROS1, PPAR-&gamma, , PPAR-&alpha,

中图分类号:

R285.5

王茜，张一昕*，石铖，郝蕾，韩雪，郭秋红，张晓栋. 楮实子对药物性肝损伤大鼠氧化应激因子的影响[J]. 天然产物研究与开发, 2019, 31(9): 1617-1623.

WANG Xi，ZHANG Yi-xin*，SHI Cheng，HAO Lei，HAN Xue，GUO Qiu-hong，ZHANG Xiao-dong. Effect of Broussonetiae Fructus on oxidative stress factors in rats with drug-induced liver injury[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2019, 31(9): 1617-1623.

[1]	许倩倩, 张咪, 庞良芳, 刘雅瑞, 李亮霞, 鄢家艳, 卢圆圆. 金樱子内生真菌Diaporthe sp. JH-1的化学成分及生物活性研究[J]. 天然产物研究与开发, 2024, 36(增刊1): 22-27.
[2]	代德财, 张笑笑, 徐晓娟, 李子昂, 董凌嫣, 陈青霞, 张蕊. 黄毛榕中具有α-葡萄糖苷酶抑制活性化合物的研究[J]. 天然产物研究与开发, 2024, 36(9): 1537-1541.
[3]	李茹, 陈雪林, 王寒蕾, 张振男, 赵霞, 张昆, 张玉梅. 水杨梅根的化学成分及α-葡萄糖苷酶抑制活性研究[J]. 天然产物研究与开发, 2024, 36(8): 1307-1319.
[4]	梁琳, 袁玺, 冼小雅, 周先丽, 王力生, 邝彤东, 梁成钦. 桂林会仙湿地真菌Talaromyces sp. W21的化学成分及其抗炎活性研究[J]. 天然产物研究与开发, 2024, 36(7): 1175-1181.
[5]	胡童霞, 张楠, 朱鑫麗, 曾彬, 吴帆, 李红亮. 荞麦蜂花粉多酚对α-淀粉酶的抑制作用[J]. 天然产物研究与开发, 2024, 36(6): 930-937.
[6]	张梦娇, 邝天浩, 赵金诺, 朱婷, 彭娟娟. 华泽兰内生真菌Septoriella phragmitis次级代谢产物及其生物活性研究[J]. 天然产物研究与开发, 2024, 36(6): 986-992.
[7]	普元柱, 陈海丰, 苏灿. 灯盏细辛总咖啡酰奎宁酸调控PI3K/Akt/FoxO3a通路延缓衰老的作用机制研究[J]. 天然产物研究与开发, 2024, 36(5): 737-747.
[8]	戴劲, 宋仕廉, 朱桂宁, 陈楠, 殷国平. 黄芪甲苷对高剂量S-氯胺酮诱导PC12细胞损伤的保护作用[J]. 天然产物研究与开发, 2024, 36(4): 669-674.
[9]	韩维维, 王博, 钟晴, 张蓉, 徐驰. 18β-甘草次酸通过PGK1糖酵解途径抑制oxLDL诱导的血管内皮细胞凋亡研究[J]. 天然产物研究与开发, 2024, 36(3): 478-484.
[10]	田梦芝, 龙佳欣, 陈笑一, 陈惠媚, 金泽龙, 李思特, 谢明霞, 杜可. β-烟酰胺单核苷酸对氧糖剥夺PC12细胞自噬的影响[J]. 天然产物研究与开发, 2024, 36(2): 260-267.
[11]	王小兰, 石静亚, 李孟, 王滢清, 郑晓珂, 冯卫生. 怀菊花茎叶中聚炔类化合物对ox-LDL诱导的HUVECs细胞损伤的保护作用[J]. 天然产物研究与开发, 2024, 36(10): 1742-1750.
[12]	郑蓉慧, 孙冬梅, 段志文, 刘晓霞, 冯涌微, 位翠杰, 陈向东, 李振雨. 基于化学模式识别的桃仁及其炮制品差异性研究[J]. 天然产物研究与开发, 2024, 36(10): 1751-1760.
[13]	姜红宇, 吕敬崑, 邵金华, 陈小明. 地卷柏的化学成分及其α-葡萄糖苷酶抑制活性研究[J]. 天然产物研究与开发, 2024, 36(1): 37-43.
[14]	白娟, 张琰, 洪术霞, 史金平, 徐丽婷, 王芳. 基于网络药理学和体外细胞实验验证探讨乳香治疗急性肾损伤的作用机制[J]. 天然产物研究与开发, 2023, 35(9): 1613-1623.
[15]	康永锋, 武改芳, 李立, 甘建红. 南海海绵Dactylospongia elegans的二倍半萜类化学成分及抗炎活性研究[J]. 天然产物研究与开发, 2023, 35(8): 1357-1363.

楮实子对药物性肝损伤大鼠氧化应激因子的影响

Effect of Broussonetiae Fructus on oxidative stress factors in rats with drug-induced liver injury

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价