天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (11): 1864-1872.doi: 10.16333/j.10016880.2019.11.003

• 研究论文 • 上一篇    下一篇

基于HPLC指纹图谱技术的地黄块根抗氧化活性谱效关系研究

耿晓桐1，王丰青3，谢彩侠1,2*，张苗1，陈志红1，龚海燕1，雷敬卫1，张重义2*   

  1. 1河南中医药大学药学院，郑州450046；2福建农林大学作物科学学院作物科学学院，福州350000；3河南农业大学农学院，郑州450000
  • 出版日期:2019-11-28 发布日期:2019-12-16
  • 基金资助:

    基金项目：国家重点研发计划（2017YFC1700705）；河南省科技攻关计划（182102311156）

Study on the relationship between antioxidant activity of Rehmannia root and spectral effect based on HPLC fingerprint technology

GENG Xiaotong1,WANG Fengqing3,XIE Caixia1,2*,ZHANG Miao1,CHEN Zhihong1,GONG Haiyan1,LEI Jingwei1,ZHANG Zhongyi2*   

  1. 1School of Medicine,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China;2College of Crop Science,Fujian Agriculture and Forestry University,Fuzhou 350000,China;3College of Agronomy,Henan Agricultural University,Zhengzhou 450000,China
  • Online:2019-11-28 Published:2019-12-16

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摘要： 本文旨在比较地黄块根不同组织及不同极性部位抗氧化活性的差异，初步分析其抗氧化活性谱效关系。利用DPPH法对855和北京1号两个地黄品种块根的菊花心（木质部）、非菊花心（韧皮部）及整体块根不同极性部位（氯仿、正丁醇和乙酸乙酯）的体外抗氧化活性进行评价，并利用Pearson相关系数法对抗氧化活性较强部位的HPLC指纹图谱与其抗氧化活性进行谱效关系分析。结果表明，地黄块根菊花心、非菊花心和整体块根的不同极性部位均有较强的抗氧化活性，其中地黄菊花心的整体抗氧化能力较弱；三个组织中抗氧化活性最强的均为乙酸乙酯部位，单因素方差分析结果显示乙酸乙酯部位与氯仿、正丁醇部位抗氧化活性的IC50值具有显著性差异；样品乙酸乙酯部位的HPLC指纹图谱共标定了14个共有峰，谱效关系分析表明，毛蕊花糖苷具有一定的抗氧化活性，色谱峰X1、X 2、X10和乙酸乙酯部位的抗氧化性具有显著相关性。该研究为地黄块根抗氧化活性药效物质成分的筛选及阐明地黄菊花心与地黄质量的相关性提供理论依据。

关键词: 地黄块根, 不同极性部位, 体外抗氧化活性, HPLC, 谱效关系

Abstract: In this study,the antioxidant activity in different tissues and different polar parts of R. glutinosa was compared and preliminary analysis of its antioxidant activity spectrum relationship.We evaluted the antioxidant activity in different polar position(chloroform, n-butanol and ethyl acetate) of radial striation(xylem),unradial striation(phloem) and whole roots of two R. glutinosa germplasms(855,Beijing1) by the method of DPPH.And using Pearson correlation coefficient method to analyze the spectral effect relationship between HPLC fingerprint and antioxidant activity of strong antioxidant activity fraction.The results showed that the radial striation,unradial striation and whole root tuber of Rehmannia glutinosa root had strong antioxidant activity,in which the overall antioxidant capacity of the radial striation was weak,and the most active was the ethyl acetate part.The results of oneway ANOVA showed significant differences in IC50 values between ethyl acetate fraction and the antioxidant activities of chloroform and n-butanol fractions.HPLC fingerprints of the ethyl acetate fraction of the sample were calibrated with 14 common peaks.The results of spectral efficiency analysis showed that the acteoside had certain antioxidant activity,and the peaks X1,X 2, X10 and ethyl acetate samples had significant correlation with oxidation resistance.This study provides a theoretical basis for the screening of antioxidative activity pharmacological components of Rehmannia glutinosa and the clarification of the relationship between the radial striation of Rehmannia glutinosa and the quality of Rehmannia glutinosa.

Key words: Rehmannia root, different polar parts, In vitro antioxidant activity, hplc, spectral effect relationship

中图分类号: 

R284.1

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