关键词: 星点设计效应面法, 大孔树脂, 还原型萝卜硫苷, 抗氧化

Abstract: This study was to investigate the optimal purification process for macroporous resins of glucoraphasatin and the antioxidation.The best type of macroporous resin was selected by 8 different types of macroporous resins for the specific adsorption amounts,adsorption and elution rates of reduced glucoraphasatin,the purification technology for glucoraphasatin was optimized by single factor investigation and central composite designresponse surface methodology.And the antioxidant activity of glucoraphasatin extractive purification before and after was characterized by the scavenging capacity of DPPH and ABTS⁺· free radicals.The results show that HPD722 macroporous resin has the best effect on purifying glucoraphasatin,the optimal condition was as follows as the sample solution pH is 5.3,flow rate is 2.5 BV/h,the concentration is 0.53 mg/mL,the eluent was 70% ethanol solution,the eluent volume was 2.5 BV and the flow rate was 1.5 BV/h.The purity increased by 44.35 times from 0.404% to 17.903%.Compared with the radish extract,the ability of the purified glucoraphasatin extractive to scavenge DPPH free radicals and ABTS⁺· free radicals increased by 67.31 and 45.27 times,respectively.

Key words: central composite designresponse surface methodology, macroporous resin, glucoraphasatin, antioxidant