天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (1): 95-102.doi: 10.16333/j.1001-6880.2020.1.014

• 开发研究 • 上一篇    下一篇

黑毛组6种石斛药效成分分析及营养价值评价

王彦兵1,2,周侯光1,尹红星1,李庆聪1,王晓媛1,2 *,李守岭1 *   

  1. 1云南省德宏热带农业科学研究所,瑞丽 678600;2广西大学农学院,南宁 530004
  • 出版日期:2020-01-28 发布日期:2020-03-25
  • 基金资助:

    农业农村部物种品种资源保护(热带作物)项目(15181301354052710);云南省现代农业橡胶产业技术体系建设(2019KJTX008-04)

Effective components analysis and nutritional value evaluation of 6 species of Dendrobium in Sect. Formosae

WANG Yan-bing1,2,ZHOU Hou-guang1,YIN Hong-xing1,LI Qing-cong1,WANG Xiao-yuan1,2 *,LI Shou-ling1 *   

  1. 1Dehong Tropical Agriculture Institute of Yunnan,Ruili 678600,China;2College of Agriculture,Guangxi University,Nanning 530004,China
  • Online:2020-01-28 Published:2020-03-25

摘要: 对石斛兰属黑毛组6种石斛进行药效成分测定及营养价值评价,为石斛种质资源鉴定、分类及药材应用提供科学依据。选取黑毛组6种石斛为实验材料,分别采用硫酸-苯酚法、热浸法、气相色谱法和酸水解法-氨基酸分析仪测定多糖、醇溶性浸出物、石斛碱和17种氨基酸含量。黑毛组6种石斛中多糖含量介于0.59%~17.08%,含量最高的翅梗石斛与含量最低的长距石斛相差28.95倍,均低于药典规定铁皮石斛多糖不得少于25%的标准;醇溶性浸出物含量介于7.62%~13.54%,均高于药典中规定铁皮石斛不少于6.5%的标准,含量最高的翅萼石斛为标准的两倍;6种石斛样品中均未检出石斛碱;6种石斛均含有色氨酸以外的17种氨基酸,总量介于3.45%~4.53%之间;6种石斛氨基酸比值系数分(SRC)介于52.42~83.74,除处子石斛外其余石斛SRC值均高于70;统计描述分析表明,6种石斛中多糖变异系数最大(137.24%),其次为醇溶性浸出物(18.95%);相关性分析表明,营养成分之间存在一定显著相关性(P < 0.05),而药效成分与营养成分及药效成分之间无显著相关性。黑毛组6种石斛氨基酸含量丰富,可提供高品质蛋白,药效成分差异显著,表明其药理作用不同,应根据成分和含量差异有针对性地开展生产和应用。本研究对药用石斛良种选育和石斛资源的开发具有一定的指导意义。

关键词: 黑毛组石斛, 药效成分, 营养价值, 相关性分析, 资源开发

Abstract: In order to provide scientific basis for the identification,classification and application of dendrobium germplasm resources,the medicinal components of 6 species of dendrobium were determined and their nutritional value was evaluated.Selecting 6 species of dendrobium in Sect. Formosae as materials,the contents of polysaccharides,alcohol-soluble extracts,dendrobine and 17 kinds of amino acids were determined by sulfuric acid-phenol method and anino acid analyzer analysis,thermal leaching method,gas chromatography and acid hydrolysis method respectively.The contents of polysaccharides was between 0.59%~17.08%,alcohol-soluble extract was between 7.62%~13.54%,dendrobine was not detected in all samples;all of dendrobiums contain 17 amino acids other than tryptophan,the total amount is between 3.45% and 4.53%.Amino acid ratio coefficients (SRC) of 6 species of Dendrobium ranged from 52.42 to 83.74.The statistical description and analysis showed that the polysaccharide variation coefficient of 6 species of Dendrobium was the largest (137.24%),followed by the alcohol-soluble extracts (18.95%).The correlation analysis showed that there was some significant correlation between the nutritional components,but no significant correlation between the medicinal components and the nutritional components and the medicinal components.6 species of Dendrobium in Sect. Formosae were rich in amino acids,which could provide high quality protein.The difference of pharmacodynamic components was significant,suggesting that their pharmacological effects were different,production and application should be carried out according to the difference of composition and content.This study has guiding significance to the breeding of excellent species of Dendrobium and the exploitation of Dendrobiumresources.

Key words: Sect. Formosae, effective components, nutritional value, correlation coefficient, resource exploitation

中图分类号: 

TS201.4 R284.1