天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (1): 127-135.doi: 10.16333/j.1001-6880.2020.1.018

• 开发研究 • 上一篇    下一篇

均质剪切辅助响应面法提取扛板归总黄酮及其抗氧化性能研究

黄国霞1,3,4*，李桥换2，阎柳娟1,3,4   

  1. 1广西科技大学 生物与化学工程学院，柳州 545006；2柳州市妇幼保健院，柳州 545000；3广西科技大学 广西高校糖资源加工重点实验室；4广西糖资源绿色加工重点实验室（广西科技大学），柳州 545006
  • 出版日期:2020-01-28 发布日期:2020-03-25
  • 基金资助:

    广西科技大学科学基金（校科自1419201）

Optimization of extraction process of total flavonoids in Polygonum perfoliatum L. using response surface methodology and high-speed shearing technique and study on its antioxidant activity

HUANG Guo-xia1,3,4*,LI Qian-huan2,YAN Liu-juan1,3,4   

  1. 1Department of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006，China;2Liuzhou Maternity and Child Healthcare Hospital,Liuzhou 545000，China;3Guangxi University of Science and Technology Guangxi Key Laboratory of Green Processing of Sugar Resources;4Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes,Guangxi University of Science and Technology,Liuzhou 545006，China

  • Online:2020-01-28 Published:2020-03-25

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摘要： 为了研究扛板归中总黄酮的最佳提取工艺及其体外抗氧化活性，在高剪切及回流提取单因素的基础上，采用响应面法考察了回流温度、回流时间、料液比、乙醇浓度对提取率的影响。 结果显示，扛板归总黄酮最优提取工艺为：回流温度79.86 ℃，回流时间2.03 h，乙醇浓度51.64%，料液比1∶59（m∶V），剪切次数7次，剪切温度50 ℃。最优工艺下总黄酮的提取率为8.37%。提取的总黄酮抗氧化性能良好，浓度为8 mg/mL时，对DPPH·、O2-·和·OH三种自由基的清除率在68%~90%之间。

关键词: 扛板归, 黄酮, 高剪切, 抗氧化

Abstract: In this paper,the optimum extraction process and antioxidant activity in vitro of total flavonoids from Polygonum perfoliatum L. was researched.The effects of extraction temperature,extraction time,ratio of material to liquid and ethanol concentration on extraction rate were investigated by single factor and response surface methodology.According to the experimental results,the optimized extraction conditions were got,which were listed as following,extraction temperature was 79.86 ℃,extraction time was 2.03 h,ethanol concentration was 51.64%,ratio of material to liquid was 1∶59,shear times was 7 times,shear temperature was 50 ℃,and the extraction rate of flavonoids under the above conditions was 8.37%.In addition,the in vitro antioxidant activities of the total flavonoids was investigated by measuring clearance abilities of O2-·,1,1-diphenyl-2-trinitrophenylhydrazine (DPPH·) and ·OH.The results showed that the total flavonoids had good antioxidant activity.When the concentration of total flavonoids was 8 mg/mL,the scavenging rates of DPPH·, O2-· and·OH were between 68%-90%.

Key words: Polygonum perfoliatum L., flavonoids, high shear, antioxidant

中图分类号: 

R284.2

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