天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (2): 224-231.doi: 10.16333/j.1001-6880.2020.2.006

• 研究论文 • 上一篇    下一篇

鱼皮胶原蛋白肽复方制品对小鼠免疫功能的影响

石举然1,李丽杰1*,张曾亮2,王敏1,卢赛1,杨迪1,王鲁慧1,邹圣灿1   

  1. 1颐海产业控股有限公司,青岛 266100;2内蒙古医科大学中医学院,呼和浩特 010110
  • 出版日期:2020-02-29 发布日期:2020-04-23
  • 基金资助:

    科技部国家重点研发计划(2018YFC0311203)

Effect of compound product of fish skin collagen on mouse immunity

SHI Ju-ran1,LI Li-jie1*,ZHANG Zeng-liang2,WANG Min1,LU Sai1,YANG Di1,WANG Lu-hui1,ZOU Sheng-can1   

  1. 1Yihai Industry Holding Co.,Ltd.,Qingdao 266100,China;2College of Traditinal Chinese Medicine,Inner Mongolia Medical University,Hohhot 010110,China
  • Online:2020-02-29 Published:2020-04-23

摘要: 本试验旨在研究鱼皮胶原蛋白肽与壳寡糖、人参提取物、黄芪提取物、当归提取物的药物组合物(鱼皮胶原蛋白肽复方制品)对免疫功能的影响。将336只SPF级健康ICR雌性小鼠随机分为四批(N = 84),分别进行细胞免疫功能、脏器/体重比值及体液免疫功能、单核-巨噬细胞功能及NK细胞活性检测。每批随机分为7组(n = 12),阴性对照组、高(3.0 g/kg)、中(1.0 g/kg)、低(0.5 g/kg)剂量复方制品组和鱼皮胶原蛋白肽组,连续灌胃30天后,测定各项功能指标。结果发现,与阴性对照组相比,各剂量组小鼠的迟发变态反应、脏器/体重比值、抗体生成细胞数无显著差异(P>0.05);复方制品各剂量组小鼠的淋巴细胞增殖能力、血清溶血素水平以及单核-巨噬细胞碳廓清吞噬指数明显增强,且效果优于鱼皮胶原蛋白肽组。由此得出,鱼皮胶原蛋白肽复方制品具有较好的增强小鼠免疫力的作用。

关键词: 鱼皮胶原蛋白肽, 复方制品, 小鼠, 免疫功能

Abstract: In order to study the effect of a compound,which comprises fish skin collagen peptide,chitosan oligosaccharide (COS),Panax ginsengextract,Astragalus extract and Angelica extract,on the mice immunity,we divided the 336 SPF healthy ICR female mice into four batches(N= 84) randomly.And the cellular immune function,organ/body weight ratio and humoral immune,monocyte-macrophage function and NK cell activity of mice were studied respectively.Each batch was divided into seven groups(n= 12),including negative control group,low-dose(0.5 g/kg),medium-dose(1.0 g/kg) and high-dose compound products group (3.0 g/kg),low-dose (0.5 g/kg),medium-dose(1.0 g/kg) and high-dose fish skin collagen peptide group(3.0 g/kg).Functional indexes were measured according to the daily continuous oral gavage for 30 days.As a result,compared with the negative control group,there were no significant differences in delayed allergy(DTH),organ/body weight ratio,and the number of antibody-producing cells between the experimental groups(P> 0.05).The proliferation ability of lymphocyte,serum hemolysin level and carbon clearance index of monocyte- macrophage were significantly enhanced in compound products group,and the effect of compound on mice immunity was better than that in the fish skin collagen peptide group.In a nutshell,the compound product of fish skin collagen peptide in this study has a better effect on enhancing the mouse immunity than fish skin collagen peptide group.

Key words: fish skin collagen peptide, compound products, mice, immune function

中图分类号: 

R965