天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (2): 257-268.doi: 10.16333/j.1001-6880.2020.2.010

• 研究简报 • 上一篇    下一篇

基于HPLC-Q-Exactive Orbitrap/MS技术分析不同剂型太子参的差异化学成分

华愉教,谢芬,周少丹*,王敏伟,仰晓云,陆元杰   

  1. 江南大学附属医院药学部,无锡 214000
  • 出版日期:2020-02-29 发布日期:2020-04-23
  • 基金资助:

    江南大学附属医院(无锡市第四人民医院)梯队人才计划(2018-21)

Difference of chemical compositions in Pseudostellariae Radix with different dosage form by HPLC-Q-Exactive Orbitrap/MS

HUA Yu-jiao,XIE Fen,ZHOU Shao-dan*,WANG Min-wei,YANG Xiao-yun,LU Yuan-jie   

  1. Department of Clinical Pharmacy,Affiliated Hospital of Jiangnan University,Wuxi 214000,China
  • Online:2020-02-29 Published:2020-04-23

摘要: 为探讨不同剂型对太子参代谢物合成积累的影响,采用高效液相色谱-串联四级杆轨道阱质谱(HPLC-Q-Exactive Orbitrap/MS)结合多元统计分析技术对太子参传统中药饮片和配方颗粒的差异化学成分进行研究。基于HPLC-Q-Exactive Orbitrap/MS代谢组学技术,结合软件数据库搜索进行成分鉴定。采用主成分分析(PCA),偏最小二乘法判别分析(PLS-DA)和正交最小二乘法判别分析(OPLS-DA)等方法筛选和鉴定差异化学成分。结果显示,太子参传统中药饮片和配方颗粒样品中化学成分能够显著区分,在正、负离子模式下分别找到98个和52个差异化学成分,这些差异化学成分主要集中在黄酮、有机酸、脂肪酸、氨基酸类化合物中且呈现不同的变化规律,主要涉及异喹啉生物碱生物合成代谢。该结果可为揭示不同剂型对太子参代谢物合成积累的影响规律提供基础资料。

关键词: 太子参, 高效液相色谱-串联四级杆静电场轨道阱质谱(HPLC-Q-Exactive Orbitrap/MS), 差异化学成分, 传统中药饮片, 中药配方颗粒

Abstract: In order to explore the influence of different dosage forms on the synthesis and accumulation of metabolites of Pseudostellariae Radix (PR),HPLC-Q-Exactive Orbitrap/MS method combined with multivariate statistical analysis was used to analyze the different chemical components in traditional PR Chinese medicine decoction pieces and PR formula particles.Based on the metabolomics technology of HPLC-Q-Exactive Orbitrap/MS,the components were identified by software database search.Principal component analysis (PCA),partial least square discriminant analysis (PLS-DA) and orthogonal least square discriminant analysis (OPLS-DA) were used to select and identify the different chemical components.Results showed that the traditional PR Chinese medicine decoction pieces and PR formula particles can significantly distinguish,98 differential chemical compositions were found in positive ion mode,while 52 differential chemical compositions were found in negative ion mode.The differential chemical compositions are mainly concentrated in flavonoids,organic acids,fatty acids,and amino acids compounds,and present different change rules,mainly involved in the isoquinoline alkaloid biosynthesis metabolic pathways.The study can provide basic information for revealing the influence law of different dosage forms on the synthesis and accumulation of metabolites in PR.

Key words: Pseudostellariae Radix, HPLC-Q-Exactive Orbitrap/MS, differential chemical compositions, traditional Chinese medicine decoction pieces, traditional Chinese medicine formula particles

中图分类号: 

R917