天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (3): 414-419.doi: 10.16333/j.1001-6880.2020.3.009

• 研究简报 • 上一篇    下一篇

紫娟茶化学成分及其抗炎抗氧化活性研究

李明超1,桂浩鑫1,李晓蕾2,刘莹1,李宇倩1,徐天瑞1,郝倩1*   

  1. 1昆明理工大学生命科学与技术学院 云南省高校靶点药物筛选与利用重点实验室,昆明 650500;2昆明理工大学分析测试中心,昆明 650093
  • 出版日期:2020-03-28 发布日期:2020-05-12
  • 基金资助:

    国家自然科学基金(31660099);云南省科技计划(2017 FD100);云南省教育厅科学研究基金(2019Y0039)

 Study on chemical constituents and their anti-inflammatory and antioxidative activities in Zijuan tea

LI Ming-chao1,GUI Hao-xin1,LI Xiao-lei2,LIU Ying1,LI Yu-qian1,XU Tian-rui1,HAO Qian1*   

  1. 1Key Laboratory of Screening and Utilization of Targeted Drugs,Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China;2Research Center for Analysis and Mersurement,Kunming University of Science and Technology,Kunming 650093,China

  • Online:2020-03-28 Published:2020-05-12

摘要: 本文对紫娟茶的化学成分进行分离纯化及其抗炎、抗氧化活性进行考察。实验采用现代分离纯化方法,从紫娟茶中共分离得到12个化合物,根据波谱学数据进行结构鉴定为:山柰酚(1)、(+)-表儿茶素(2)、(-)-表儿茶素-3-O-没食子酸酯(3)、杨梅素(4)、表没食子儿茶素-3-O-(3″-O-甲基)没食子酸酯(5)、槲皮素(6)、没食子酸(7)、表没食子儿茶素没食子酸酯(8)、3-O-没食子酰基奎宁(9)、小木麻黄素(10)、1,4,6-三-O-没食子酰基-β-D-葡萄糖(11)、咖啡因(12),其中化合物9为首次从紫娟茶中分离得到。化合物11表现出良好的抗氧化活性,化合物1、3、4、6、8~11均具有显著的抗炎活性。

关键词: 紫娟茶, 化学成分, 抗氧化活性, 抗炎活性

Abstract: This paper reported the chemical constituents and their anti-inflammatory and antioxidative activity of biological of Zijuan tea.Zijuan tea is known for purple-colored buds,leaves,stems,calices,peduncles and tea infusion.It was bred from an individual Yunnan Daye Camellia sinensis var. assamica in Yunnan province of China.In this study,column chromatography and high-performance liquid chromatography (HPLC) analysis were used for isolation and purification,12 known compounds were isolated.The structures of them were determined by detailed analysis of their NMR spectra and chemical evidences.The known compounds were identified as kaempferol (1),quercetin (2),(-)-epicatechin-3-O-gallate (3),(+)-epicatechin (4),myricetin (5),epigallocatechin-3-O-(3″-O-methyl) gallate (6),gallic acid (7),epigallocatechin gallate (8),3-O-galloyl quinine (9),strictinin (10),1,4,6-tri-O-galloyl-β-D-glucose (11),caffeine (12),which compound 9 were the first reported from Zijuan tea.Among of them,compound 11 showed favorable antioxidant activity,and compounds 1,3,4,6,8-11 all had significant anti-inflammatory activities.

Key words: Zijuan tea, chemical composition, anti-inflammatory activity, antioxidant activity

中图分类号: 

R93