天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (5): 727-733.doi: 10.16333/j.1001-6880.2020.5.002

所属专题： No.3

• 研究论文 • 上一篇    下一篇

3种石斛多糖及其降血糖活性的比较研究

叶广英，章金辉，李杰，刘海林，王再花*   

  1. 广东省农业科学院环境园艺研究所 广东省园林花卉种质创新综合利用重点实验室，广州 510640

  • 出版日期:2020-05-28 发布日期:2020-06-16
  • 基金资助:
    广东省重点领域研发计划（2019B020214005）；广东省现代农业产业技术体系创新团队项目（粤农农函2019［1019］号）；2019年院新兴学科团队创意农业研究团队项目（201806xx）；科技创新战略专项（高水平农科院建设）（R2019PY-QY003)

Comparison of polysaccharides and its hypoglycemic activity from three Dendrobium species

YE Guang-ying,ZHANG Jin-hui,LI Jie,LIU Hai-lin,WANG Zai-hua*   

  1. Environmental Horticulture Research Institute,Guangdong Academy of Agricultural Sciences Guangdong Provincial Key Lab of Ornamental Plant Germplasm Innovation and Utilization,Guangzhou 510640,China
  • Online:2020-05-28 Published:2020-06-16

PDF (PC)

279

摘要：

天然多糖安全、低毒，是潜在糖尿病治疗药物。为寻找活性高、含量高的植物多糖资源，本文从多糖得率、结构和降血糖活性三个方面，对云南地方药材大苞鞘石斛、铁皮石斛和金钗石斛进行比较。通过热水浸提、透析和层析得到多糖，然后用糖尿病小鼠模型测定降血糖活性。结果表明大苞鞘石斛的多糖得率高（14.1%，DW），且分子量较小，其单糖组成与铁皮石斛多糖相似；降血糖效果强于铁皮石斛和金钗石斛多糖。大苞鞘石斛可作优良的亲本，为降血糖的石斛多糖资源开发提供育种资源。

关键词: 大苞鞘石斛, 天然多糖, 提取, 纯化, 降血糖活性

Abstract:

Natural polysaccharides are safe and low-toxic,and are potential drugs for the treatment of diabetes.In order to find plant polysaccharide resources with high activity and high content,Dendrobium wardianum Warner,D. officinale and D. nobile were compared in terms of yield,structure and hypoglycemic activity of polysaccharides.Polysaccharides were obtained by hot water leaching,dialysis and chromatography,and then the hypoglycemic activity was measured using a diabetic mouse model.The results showed that D. wardianum had a highest polysaccharide yield (14.1%,DW).DWCP had the lowest molecular weight and its monosaccharide composition is similar to DOPP.The hypoglycemic activity of DWCP was significantly better than the other two polysaccharides.It indicated that D. wardianum could be used as an excellent parent,which provides breeding resources for the development of hypoglycemic polysaccharide resources.

Key words: Dendrobium wardianum, polysaccharide, extraction, purification, hypoglycemic activity

中图分类号:  R931.2

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