天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (5): 845-850.doi: 10.16333/j.1001-6880.2020.5.018

• 开发研究 • 上一篇    下一篇

鳞柄小奥德蘑多糖对巨噬细胞免疫功能的调节作用

王晶1,2,3*，史琪1，随晶晶1，常世民1,2,3，杜娟2，闫训友1,2,3   

  1. 1廊坊师范学院生命科学学院；2河北省食药用菌资源高值利用技术创新中心；3廊坊市细胞工程与应用研究重点实验室，廊坊 065000

  • 出版日期:2020-05-28 发布日期:2020-06-16

Immunomodulatory effect of polysaccharide from Oudemansiella furfuracea on murine macrophages

WANG Jing1,2,3*,SHI Qi1,SUI Jing-jing1,CHANG Shi-min1,2,3,DU Juan2,YAN Xun-you1,2,3   

  1. 1College of Life Sciences,Langfang Normal University;2Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province;3Langfang Key Laboratory of Cell Engineering and Application,Langfang 065000,China

  • Online:2020-05-28 Published:2020-06-16

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摘要：

研究鳞柄小奥德蘑多糖对小鼠巨噬细胞的免疫调节作用。采用灌洗腹腔法收集小鼠巨噬细胞，建立其体外培养体系；采用鸡血红细胞法、荧光探针标记、总一氧化氮检测和酶联免疫吸附试验等方法分别检测巨噬细胞吞噬能力、NO合成量、肿瘤坏死因子-α、白细胞介素-1、白细胞介素-6和白细胞介素-12等的分泌量。结果显示，与空白对照组相比，鳞柄小奥德蘑多糖能显著增强体外培养和腹腔内巨噬细胞的吞噬能力和NO合成量，增加体外培养的小鼠巨噬细胞对肿瘤坏死因子-α、白细胞介素-1、白细胞介素-6和白细胞介素-12等细胞因子的分泌量。因此，鳞柄小奥德蘑多糖可能通过提高细胞对NO和多种免疫相关信号分子的分泌量，增强细胞的吞噬能力，进而调节小鼠巨噬细胞的免疫功能。

关键词: 鳞柄小奥德蘑, 多糖, 巨噬细胞, 细胞因子, 免疫调节

Abstract:

To investigate the immunomodulatory effects of polysaccharide from Oudemansiella furfuracea on murine macrophages in vivo and in vitro.Peritoneal macrophages were collected using peritoneal lavage method and the culture system was established.Chick red blood cell phagocytosis test,fluorescent probe technique,total nitric oxide assay and enzyme-linked immunosorbent assay were carried out to measure phagocytic activity,the content of nitric oxide,the levels of tumor necrosis factor-α,interleukin-1,interleukin-6 and interleukin-12 in culture supernatant,respectively.The results showed that polysaccharide from O. furfuracea in the range of certain concentration enhanced the phagocytosis ability and NO content of murine macrophages in vivo and cultured in vitro,and improved the secretion level of tumor necrosis factor-α,interleukin-1,interleukin-6 and interleukin-12 in culture supernatant,compared with control group.So,the immune function of murine macrophages could be modulated by polysaccharide from O. furfuracea at a certain concentration.

Key words: Oudemansiella furfuracea, polysaccharide, murine macrophages, cytokine, immunomodulation

中图分类号:  TS201.4 S646.9

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