Jiaosu contains a variety of microorganisms.In order to explore the microbial community composition and antioxidant activity of different Jiaosu,the pH value and total phenol content of pitaya,blueberry and mulberry Jiaosu were determined.The antioxidant activity was analyzed by DPPH free radical and hydroxyl radical scavenging ability.The structure of bacterial and fungal communities was analyzed by high-throughput sequencing.The results showed that the total phenol content and antioxidant activity of blueberry Jiaosu in the three Jiaosu was the highest and the pH value was the lowest.A total of 2122 bacterial OTUs and 2592 fungal OTUs were obtained from the three Jiaosu.The α-diversity analysis showed that the diversity of bacterial community was higher than that of fungal community.Proteobacteria and Ascomycota are the dominant phylum of the three Jiaosu,and the abundance of Mesorhizobium and Hanseniaspora was higher and different.The composition of microbial community was correlated with pH value,total phenol content,hydroxyl radical scavenging capacity and DPPH radical scavenging capacity.The results of this study provided a theoretical reference for the development and optimization of functional Jiaosu products.