天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (6): 1000-1005.doi: 10.16333/j.1001-6880.2020.6.013

• 研究简报 • 上一篇    下一篇

黄草乌内生真菌接骨木镰孢菌B10.2次生代谢产物研究

罗萍,舒燕,朱丽,丁中涛,蔡乐*   

  1. 云南大学 云南省高校功能分子分析与生物转化重点实验室;教育部自然资源药物化学重点实验室;化学科学与工程学院,昆明 650091

  • 出版日期:2020-06-28 发布日期:2020-07-06
  • 基金资助:
    国家自然科学基金(81960760)

Secondary metabolites of Fusarium sambucinum B10.2 from Aconitum vilmorinianum Kom.

LUO Ping,SHU Yan,ZHU Li,DING Zhong-tao,CAI Le*   

  1. Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province;Key Laboratory of Medicinal Chemistry for Natural Resource,Ministry of Education;School of Chemical Science and Technology,Yunnan University, Kunming 650091,China

  • Online:2020-06-28 Published:2020-07-06

摘要:

本文对分离自黄草乌(Aconitum vilmorinianum Kom.)的一株接骨木镰孢菌(Fusarium sambucinum B10.2)的次生代谢产物进行了研究,从中分离鉴定了10个化合物:cyclonerodiol(1)、trichoderiol A(2)、T-2 tetraol(3)、sambacide(4)、12β-acetoxy-4,4-dimethyl-24-methylene-5α-cholesta-8,14-diene-3β,11α-diol(5)、bisphenol A(6)、β-谷甾醇(7)、木兰脂素(8)、吲唑(9)、尿嘧啶(10),1~3和5~10均是首次从接骨木镰孢菌中分离得到,其中6首次从天然产物中发现。活性研究表明6和9对金黄色葡萄球菌具有较强的抑制活性,其最小抑菌浓度(MIC)与阳性对照相同,为64 μg/mL;4对大肠杆菌的生长具有一定的抑制作用,最小抑菌浓度(MIC)为32 μg/mL;2、3和4具有中等强度的乙酰胆碱酯酶抑制活性。

关键词: 接骨木镰孢菌, 黄草乌, 次生代谢产物, 抑菌活性, 乙酰胆碱酯酶抑制活性

Abstract:

Ten compounds were obtained from Fusarium sambucinum B10.2,which was isolated from Aconitum vilmorinianum Kom.Their structures were identified as:cyclonerodiol (1),trichoderiol A (2),T-2 tetraol (3),sambacide (4),12β-acetoxy-4,4-dimethyl-24-methylene-5α-cholesta-8,14-diene-3β,11α-diol (5),bisphenol A (6),β-sitosterol (7),magnolin (8),indazole (9),uracil (10).Compounds 1-3,5-10 were isolated from this fungus for the first time,and 6 was first obtained from nature.The results of bioactivity assays showed that compound 4 could inhibit the growth of Escherichia coli with the minimum inhibitory concentration (MIC) of 32 μg/mL.Compounds 6 and 9 showed strong inhibitory activity against Staphylococcus aureus,and the MIC was 64 μg/mL,which was at the same grade as positive control.Compounds 2,3 and 4 exhibited moderate acetylcholinesterase (AChE) inhibitory effects.

Key words: Fusarium sambucinum, Aconitum vilmorinianum, secondary metabolites, antibacterial 

中图分类号:  R284.2