天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (6): 1006-1013.doi: 10.16333/j.1001-6880.2020.6.014

• 研究简报 • 上一篇    下一篇

基于UPLC-ESI-IT-TOF-MS方法对血府逐瘀汤中8个成分同时含量测定

傅春燕1,2*,王惠余2,刘永辉3,王杨4   

  1. 1中南大学化学化工学院,长沙 410083;2邵阳学院药学院和食品与化学工程学院;3邵阳学院附属第二医院,邵阳 422000;4中南大学湘雅医院中西医结合研究所,长沙 410008

  • 出版日期:2020-06-28 发布日期:2020-07-06
  • 基金资助:
    国家自然科学基金(21675174,21873116,81673719);2018年度湖南省技术创新引导计划临床医疗技术创新引导项目(2018SK51902)

Simultaneous determination of eight components in Xuefu Zhuyu Decoction based on UPLC-ESI-IT-TOF-MS method

FU Chun-yan1,2 *,WANG Hui-yu2,LIU Yong-hui3,WANG Yang4   

  1. 1College of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China;2College of Pharmacy and College of Food and Chemical Engineering,Shaoyang University;3The Second Affiliated Hospital,Shaoyang University,Shaoyang 422000,China;
    4 Institute of Integrated Traditional Chinese and Western Medicine,Xiangya Hospital,Central South University,Changsha 410008,China

  • Online:2020-06-28 Published:2020-07-06

摘要: 建立UPLC-ESI-IT-TOF-MS法测定血府逐瘀汤中苦杏仁苷、羟基红花黄色素A、甘草苷、芦丁、芸香柚皮苷、柚皮苷、新橙皮苷和柴胡皂苷A 8个成分含量的方法。采用Shim-Pack XR-ODS C18色谱柱(2.0 mm ×75 mm,1.6 μm),以水/甲酸(0.1%)-乙腈为流动相,梯度洗脱;采用ESI离子源,正负离子模式同时采集,IT-TOF-MS检测器定性定量测定。结果表明,8个成分的线性关系、日内和日间精密度、稳定性均良好,加样回收率在89.8%~108.1%范围内。本实验方法简单、灵敏,充分发挥了IT-TOF-MS检测器稳定性好、质量数精确、分辨率高、准分子离子外标法定量准确的优势,为血府逐瘀汤的质量控制提供科学依据。


关键词: 血府逐瘀汤, 8个成分, UPLC-ESI-IT-TOF-MS, 质量控制

Abstract:

To establish a UPLC-ESI-IT-TOF-MS method for simultaneous determination of amygdalin,hydroxysafflor yellow A,liquiritin,rutin,narirutin,niringin,neohesperidin and saikosaponin A in Xuefu Zhuyu Decoction.The chromatographic separation was on Shim-Pack XR-ODS C18 column (2.0 mm ×75 mm,1.6 μm) with a mobile phase consisting of water/ formic acid (0.1%)-acetonitrile and gradient elution.ESI ionization source,combined with positive and negative scanning switch simultaneously,and IT-TOF-MS was used for qualitative and quantitative analysis.The results indicated that the linear relationship,intra-day and inter-day precision,and stability of the eight components were all good,and the average recoveries of the eight components were in the range of 89.8%-108.1%.This experimental method is simple and sensitive.which gives full play to the advantages of the IT-TOF-MS detector with good stability,accurate mass,high resolution,and accurate quantification by the external standard method of the excimer ion,and can be expected to provide a scientific basis for quality control of Xuefu Zhuyu Decoction.

Key words: Xuefu Zhuyu Decoction, eight components, UPLC-ESI-IT-TOF-MS, quality control

中图分类号:  R917