To study the anti-tumor effect of sanggenon D in vivo and in vitro.The in vitro method was first to co-culture three types of cancer cells (human liver cancer HEPG2 cells，mouse melanoma B16F0 cells,and mouse melanoma B16F10 cells) with sanggenon D solution.Then,SRB method (sulforhodamine B) was used to investigate the effect of sanggenon D on the proliferation of tumor cells,and IC₅₀ was calculated.The effects of different concentrations of sanggenon D on melanoma cells were investigated.The in vivoexperimental method was to establish a mouse H22 tumor model,and to investigate the inhibitory effect of the drug on tumor growth by intraperitoneal injection of 12.5,25 and 50 mg/kg of sanggenon D.The results showed that sanggenon D inhibited the proliferation of B16F0,B16F10 and HepG2 cells,among which HepG2 cells were the most sensitive to sanggenon D,and IC₅₀ was calculated to be 22.61±0.26 μmol/L The inhibitory effect on the cells was enhanced with the increase of the concentration of sanggenon D,and the difference was significant (P<0.01).sanggenon D promotes melanin production in B16F0 cells and promotes cell differentiation.The inhibitory rate of H22 transplanted tumor in mice at 12.5,25 and 50 mg/kg was 32.51% to 45.72%.There was no significant difference in the changes of body weight and organ index in the mice treated with sanggenon D compared with the blank group and the model group.This indicated that sanggenon D could inhibit the growth of transplanted tumor and the proliferation of tumor cells in mice,and it also indicated that sanggenon D had anti-tumor effects in vivo andin vitro.