The HPLC fingerprints and chemical pattern recognition were studied to provide new method for quality control and evaluation of Platycodins Radix. The HPLC was selected to establish the fingerprints of 15 batches Platycodins Radix from different habitats. The chromatographic separation was performed on an YMC hydrosphere C₁₈ analytical column (250 × 4.6 mm,5 μm),a mixture of water and acetonitrile solution was selected as mobile phase for gradient elution,and the detection wavelength was set as 210 nm. The chemical pattern recognition of similarity evaluation,cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were used to analyze and evaluate the quality of Platycodins Radix. The results showed that the fingerprints of 15 batches of Platycodins Radix from different habitats were successfully established,which possessed 21 common peaks and 7 common peaks could be identified using standards. The results about the chemical pattern recognition showed that the similarities of those fingerprints were between 0.927 and 0.991,and 15 batches of Platycodins Radix could be divided into 3 groups by both cluster analysis and principal component analysis. Furthermore,the quality difference of those Platycodins Radix was mainly caused by 13 common peaks according to the results of orthogonal partial least squares discriminant analysis. In a word,the fingerprints combined with chemical pattern recognition provides a simple and accurate method for the quality control and evaluation of Platycodins Radix.