The prices of commercially available vine tea vary significantly due to their different parts. Herein,an efficient and reliable method using high performance liquid chromatography was developed to study the chemical constituents differences of vine tea,HPLC separation was carried out to establish the fingerprints for leaves and buds of Ampelopsis grossedentata (vine tea) on an Agilent Poroshell 120 EC-C₁₈ column (3.0 × 100 mm,2.7 μm) gradient eluted with a mixture consisting of acetonitrile (B) and 0.05% formic acid-water (A) as the mobile phase,365 nm as the wavelength,1 μL as the injection volume,40 ℃ as the column temperature,0.4 mL/min as the flow rate. 10 common peaks were designated in leaves and 12 common peaks were designated in buds as a result. No evident difference was found in terms of component number by comparing the two fingerprints. Principle component analysis (PCA),orthogonal partial least squares discriminant analysis (OPLS-DA) and hierarchical clustering analysis (HCA) indicated the different metabolic profiles between the two parts (leaves and buds). The structures confirmation of seven potential markers was conducted by ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. Among the seven markers,the contents of compounds dihydromyricetin (peak 1 of common peaks in fingerprint),isomer of dihydromyricetin (peak 2 of common peaks in fingerprint),myricetin (peak 8 of common peaks in fingerprint),unknown component (peak 10 of common peaks in fingerprint) and ellagic acid (not common peaks in fingerprint) in buds were higher,while the contents of compounds myricitrin (peak 6 of common peaks in fingerprint) and myricetin-pentoside (peak 5 of common peaks in fingerprint) in leaves were higher. It is the first report depicting the in-depth chemical differentiation among two parts of Ampelopsis grossedentata, which is beneficial to their quality control of vine tea.