天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (9): 1562-1575.doi: 10.16333/j.1001-6880.2020.9.015

• 开发研究 • 上一篇    下一篇

不同产地滇重楼须根中氨基酸类成分的UPLC分析与评价

谷文超1,2，赵顺鑫1，杨敏2，黄小兰1，黎海灵2，周浓1,2*


  

  1. 1重庆三峡学院生物与食品工程学院 三峡库区道地药材绿色种植与深加工重庆市工程实验室，重庆 404120；2大理大学药学与化学学院，大理 671000

  • 出版日期:2020-09-28 发布日期:2020-09-25
  • 基金资助:
    国家自然科学基金（81260622）；重庆市自然科学基金（cstc2018jcyjAX0267）；云南省教育厅科学研究基金（2018JS413）

Simultaneous determination of amino acids in Paris polyphylla var. yunnanensis fibril from different habitats by UPLC

GU Wen-chao1,2,ZHAO Shun-xin1,YANG Min2,HUANG Xiao-lan1,LI Hai-ling2,ZHOU Nong1,2*   

  1. GU Wen-chao1,2,ZHAO Shun-xin1,YANG Min2,HUANG Xiao-lan1,LI Hai-ling2,ZHOU Nong1,2*

  • Online:2020-09-28 Published:2020-09-25

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摘要：

建立柱前衍生-超高效液相色谱法测定滇重楼须根中15种氨基酸含量，对不同产地样品进行分析评价。采用UPLC-PDA仪器，使用ACQUITY UPLC BEH C18色谱柱（2.1 mm×150 mm，1.7 μm），流动相A为0.1 mol/L乙酸钠溶液（冰醋酸调节pH=6.5）-乙腈（97∶3），B为乙腈-水（4∶1），梯度洗脱，检测波长254 nm，流速0.2 mL/min。滇重楼须根中检出15种氨基酸线性关系良好（r≥0.999 7），平均回收率RSD均小于3.00%。各产地均以天门冬氨酸和谷氨酸含量为最高，栽培滇重楼须根氨基酸平均含量高于野生品。本研究建立了分析评价滇重楼须根中氨基酸类成分的方法，为滇重楼须根的资源开发与利用提供科学依据。



关键词: 滇重楼, 须根, 氨基酸, 栽培品, 野生品

Abstract:

To establish an analytical method for simultaneously determining the contents of 15 amino acids and analyze the content of amino acids in Paris polyphylla var. yunnanensis fibril from different places. The sample was tested by UPLC-PDA instrument, and the amino acids were separated on a ACQUITY UPLC BEH C18 column (1.7 μm，2.1 mm×150 mm) with gradient elution of 0.1 mol/L sodium acetate buffer (adjusted to pH 6.5 with acetic acid)-acetonitrile (97∶3) (A) and acetonitrile-water (4∶1) (B). UV detection wavelength was set at 254 nm and the flow rate was set at 0.2 mL/min. The kinds of 15 amino acids in P. polyphylla var. yunnanensis fibril were determined and showed a good linear relationship within the linear range (r ≥ 0.999 7),and the average recovery RSD was less than 3.00%. The content of aspartic acid and glutamic acid was the highest in all producing areas,and the average content of amino acid in the fibrous roots of cultivated P. polyphylla var. yunnanensis was higher than that of wild. In this study,we established a method to analyze and evaluate the amino acids in P. polyphylla var. yunnanensis fibrous roots,and provided a scientific basis for the development and utilization in P. polyphylla var. yunnanensis fibrous roots resources.


Key words: Paris polyphylla var. yunnanensis, fibril, amino acid, transplants, wild samples

中图分类号:  R284.1 Q946

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