Six new Chinese medicine complexes of silybin calcium,iron,zinc,selenium,cobalt and chromium were synthesized and characterized by IR,UV,LC-MS,EDTA complexometric titration and elemental analysis.The results showed that coordination ratios of these six silybin complexes were 2∶1 (silybin ratio to trace elements).The safety of complexes was evaluated by acute toxicity test on mice,and found that no obvious toxicity of these complexes in vivo.Furthermore,the anti-bacterial and anti-tumor activities were determined respectively by double dilution method and MTT assay.For the anti-bacterial assay,nine types of bacteria were used in this experiment and the minimum inhibitory concentration (MIC) were determined.The results showed that the anti-bacterial effect of silybin was increased to 2 to 4 times after reaction with the six trace elements.For the anti-tumor assay,experiments were carried out in 5 types of cancer cells and the half inhibitory concentration (IC₅₀) of the six complexes was determined.The results showed that the anti-tumor effect of these complexes was increased to 1.11-1.53 times compared with that of Silybin.In conclusion,the anti-bacterial and anti-tumor activities of silybin are elevated after the formation of complexes with the trace elements.