天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (11): 1852-1859.doi: 10.16333/j.1001-6880.2020.11.007

• 研究简报 • 上一篇    下一篇

陕西商洛艾叶精油和醇提物成分分析及其抗菌抗氧化活性研究

路露1,2，姚琪2，束成杰2，姜洪芳2，朱凯1*，石宝俊1,2*   

  1. 1南京林业大学化学工程学院，南京 210008；2南京野生植物综合利用研究院 南京 211100

  • 出版日期:2020-11-28 发布日期:2020-12-04
  • 基金资助:
    国家重点研发计划（2018YFD0600405）

Composition,antibacterial and antioxidant activities of essential oil and alcohol extract from Artemisia argyi in Shangluo,Shaanxi Province

LU Lu1,2,YAO Qi2,SHU Cheng-jie2,JIANG Hong-fang2,ZHU Kai1*,SHI Bao-jun1,2*#br#

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  1. 1College of Chemical Engineering,Nanjing Forestry University,Nanjing 210008，China;2 Nanjing Institute for Comprehensive Utilization of Wild Plants,Nanjing 211100,China

  • Online:2020-11-28 Published:2020-12-04

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摘要： 艾叶是一种临床常用的中草药，其分布广泛。为进一步深入的开发利用艾叶，本研究通过GC-MS分析了艾叶精油和艾叶醇提物的主要成分，并对比了二者的抗菌和抗氧化活性。结果表明：艾叶精油与艾叶醇提物的主要成分具有很大的差异，艾叶精油主要成分为桉叶油醇（21.11%）和龙脑（10.77%）；而艾叶醇提物主要成分为2-甲基四氢噻（20.18%）、异硫氰酸乙酯（10.47%）和二乙二醇二乙酸酯（10.06%）。艾叶精油与艾叶醇提物对5种菌种（大肠杆菌、金黄色葡萄球菌、白色念珠菌、枯草芽孢杆菌、绿脓杆菌）均具有一定的抑菌效果，其中对枯草芽孢杆菌的一直效果最佳，MIC/MBC值分别为1.95、7.81 mg/mL和3.91、7.81 mg/mL。艾叶精油和艾叶醇提物浓度为32 mg/mL时二者对DPPH 清除能力分别达到89.5%和77.6%。因此，艾叶精油的抑菌和抗氧化活性均优于艾叶醇提物。

关键词: 艾叶精油, 艾叶醇提物, 成分分析, 抑菌, 抗氧化

Abstract:

Artemisia argyi is a widely distributed and commonly used Chinese herbal medicine.For further development and utilization,the main components of essential oil and alcohol extract of Artemisia argyi were analyzed by GC-MS,and their antibacterial and antioxidant activities were compared.The results showed that the main components of Artemisia argyi essential oil and alcohol extract were very different.The main components of Artemisia argyi essential oil were eucalyptoloccupied (21.11%) and α-bornene (10.77%);while the main components of the ethanol extract of Artemisia argyi were 2-methyltetrahydrothiophen (20.18%),ethyl isothiocyanate (10.47%) and ethylene glycol diacetate (10.06%).Artemisia argyi essential oil and alcohol extracts have a certain bacteriostatic effect on 5 strains (Escherichia coli,Staphylococcus aureus,Candida albicans,Bacillus subtilis,Pseudomonas aeruginosa),among which the effect on Bacillus subtilis is the best,MIC / MBC values are 1.95,7.81 mg/mL and 3.91,7.81 mg/mL,respectively.When the concentration of essential oil and alcohol extract of Artemisia argyi were 32 mg/mL,their DPPH scavenging capacity reached 89.5% and 77.6%,respectively.Therefore,the antibacterial and antioxidant activities of essential oil from Artemisia argyi are better than alcohol extract of Artemisia argyi.

Key words: Artemisia argyi essential oil, Artemisia argyi , alcohol extract, component analysis, antibacterial, antioxidant

中图分类号:  R932 TQ65

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