Sanguisorba officinalis L.(the dried root of Sanguisorba,Rosaceae) is a traditional medicinal herb that is widely distributed in Europe and Asia.It has been officially recorded in the Chinese Pharmacopoeia (2015),possesses activities such as detoxification,analgesia and hemostasis,and has been used for the prevention and treatment of various diseases in tradition,including burns and scalds,bleeding hemorrhoids,metrostaxis,bleeding wounds,hematochezia,and swollen carbuncles.In order to better evaluate the quality of S. officinalis,the chemical constituents of the 70%EtOH extract was investigated.After physicochemical and spectral analysis,20 compounds were isolated and identified:sanguiin H-4 (1),3,4-dihydroxy-5-methoxybenzyl ester (2),gallic acid (3),methyl gallate (4),catechin (5),epicatechin (6),gallocatechin (7),epigallocatechin (8),epigallocatechin gallate (9),luteolin-7-O-β-D-glucoside (10),pomonic acid (11),2α-hydroxyl oleanolic acid (12),betulinic acid (13),oleanolic acid (14),ziyuglycoside II (15),1,4,6-tri-O-galloyl-β-D-glucopyranose (16),methyl-6-O-galloyl-β-D-glucopyranoside (17),(E)-7-hydroxy-3,7-dimethyl-2-octenyl-6-O-α-L-arabinofuranosyl-β-D-glucopyranoside (18),7-hydroxy-3,7-dimethyloctyl-6-O-α-L-arabinofuranosyl-β-D-glucopyranoside (19),n-butyl-β-D-glucopyranoside (20).Among them,compounds 9 and 20 were isolated from the genus Sanguisorba for the first time.The results provided a material basis for the quality evaluation of S. officinalis.