天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (3): 353-361.doi: 10.16333/j.1001-6880.2021.3.001

• 研究论文 •    下一篇

芦荟的HPLC指纹图谱建立、化学模式识别分析及其含量测定

严雅慧1,2，李淑萍1，热依木古丽·阿布都拉1，阿吉艾克拜尔·艾萨1,2*


  

  1. 1中国科学院大学，北京 100049；2中国科学院干旱区植物资源化学重点实验室 中国科学院新疆理化技术研究所，乌鲁木齐 830011

  • 出版日期:2021-03-28 发布日期:2021-04-01
  • 基金资助:
    中国科学院西部之光计划（2018-XBQNXZ-B-002，2017-XBQNXZ-B-005）；自治区天池博士计划（2019）

Establishment of HPLC fingerprint,chemical pattern recognition analysis and content determination of aloe

YAN Ya-hui1,2,LI Shu-ping1,ABDULA Rahima1,AISA Hajiakber1,2*   

  1. 1University of Chinese Academy of Sciences,Beijing 100049,China;2Key Laboratory of Plant Resources and Chemistry in Arid Regions Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011,China

  • Online:2021-03-28 Published:2021-04-01

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摘要： 采用Phenomenex C18色谱柱（250 mm× 4.6 mm，5 μm），以甲醇-乙腈-0.3%磷酸水为流动相进行梯度洗脱，建立芦荟的指纹图谱。运用化学模式识别方法对不同产地芦荟药材质量控制方法进行评价。结果表明：12批芦荟HPLC指纹图谱共标定23个共有峰，并通过对照品指认其中6个成分；除了广西的3批药材之外，其他药材相似度都在0.93以上；聚类分析和主成分分析将12批芦荟分为3类；利用正交偏最小二乘判别法筛选出芦荟药材差异的5个色谱峰，并以相同HPLC法对其进行含量测定。本文将HPLC 指纹图谱与化学模式识别相结合的方法简便准确，为芦荟药材的质量控制和品质评价提供依据。


关键词: 芦荟, 高效液相色谱指纹图谱, 主成分分析, 聚类分析, 正交偏最小二乘法判别分析, 含量测定

Abstract:

The fingerprint of aloe from different origins was established,and the chromatographic separation was performed on a Phenomenex C18 column (250 mm×4.6 mm,5μm),and a mixture of methannol-acetonitrile-0.3 % phosphoric acid water solution was selected as the mobile phase for gradient elution.The high-performance liquid chromatography (HPLC) fingerprint and chemical pattern recognition were studied to provide new method for quality control and evaluation of the different production areas of aloe.The results revealed that the HPLC fingerprint of aloe was calibrated with a total of 23 common peaks,and 6 common peaks could be identified using the references.The similarities of other medicinal materials was above 0.93 except for three batches of Guangxi medicinal materials and 12 batches of aloe could be divided into 3 groups by both cluster analysis and principal component analysis (PCA).Furthermore,the quality differences of aloe from different origins were mainly caused by 5 common peaks according to the results of orthogonal partial least squares discriminant analysis (OPLS-DA).Meanwhile,the contents of 6 components were determined by the same HPLC method.In brief,the establishment of HPLC fingerprint combined with the application of chemical pattern recognition could provide a simple and accurate method for the quality control and evaluation of aloe．

Key words: aloe, HPLC fingerprint, PCA, cluster analysis, OPLS-DA, content determination

中图分类号:  R282.5

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