天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (6): 935-942.doi: 10.16333/j.1001-6880.2021.6.005

• 研究论文 • 上一篇    下一篇

响应面法优化藜麦麸皮皂苷最佳提取工艺及其α-葡萄糖苷酶抑制活性

董琦1,2,3,谭亮1,胡娜1,2,王洪伦1,2*   

  1. 1中国科学院西北高原生物研究所 中国科学院藏药研究重点实验室;2青海省藏药研究重点实验室,西宁 810008;3中国科学院大学,北京 100049

  • 出版日期:2021-06-28 发布日期:2021-07-01
  • 基金资助:
    青海省科技厅项目(2019-ZJ-7100);西宁市科学技术项目(2018-Y-15)

Study on the optimization of the extraction technology by response surface methodology and inhibitory activity of α-glucoside of total saponins from the bran of Chenopodium quinoa Willd.

DONG Qi 1,2,3,TAN Liang1,HU Na1,2,WANG Hong-lun1,2*   

  1. 1CAS Key Laboratory of Tibetan Medicine Research,Northwest Institute of Plateau Biology;2Qinghai Provincial Key Laboratory of Tibetan Medicine Research,Xining 810008,China;3University of Chinese Academy of Sciences,Beijing 100049,China

  • Online:2021-06-28 Published:2021-07-01

摘要:

采用响应面法优化了藜麦麸皮超声提取皂苷的最佳工艺,并考察了最佳提取工艺条件下提取物对α-葡萄糖苷酶的抑制活性。同时建立酶反应动力学方程对其抑制动力学进行分析,探讨了藜麦麸皮提取物对α-葡萄糖苷酶的抑制机制。实验结果表明,藜麦麸皮超声提取皂苷的最佳工艺条件为液料比15 mL/g,75%乙醇超声提取,超声时间1.5 h,超声温度45 ℃,在此条件下藜麦麸皮皂苷的提取得率为2.370%±0.022%。α-葡萄糖苷酶抑制活性实验表明提取物的抑制作用明显强于阳性药阿卡波糖(P < 0.05),其IC50值为0.620±0.057 mg/mL。酶动力学研究表明藜麦麸皮总皂苷提取物是一种可逆的混合性抑制类型。本研究为明确藜麦麸皮提取物的降低餐后血糖作用机理提供了参考,为藜麦麸皮的开发与利用提供了依据。

关键词: 藜麦麸皮皂苷;响应面法;提取工艺;α-葡萄糖苷酶;抑制机制 

Abstract:

Response surface methodology was used to optimize the ultrasonic extraction process of saponins from the bran of Chenopodium quinoa Willd.and the inhibitory activity of the extract against α-glucosidase was investigated under the optimal extraction conditions.The enzyme reaction kinetics equation was established to analyze the inhibition kinetics and mechanism.The results showed that the optimal extraction conditions of saponins from quinoa bran were as follows:liquid-solid ratio of 15 mL/g,ultrasonic extraction with 75% ethanol,ultrasonic time of 1.5 h,ultrasonic temperature of 45 ℃.Under these conditions,the extraction yield of saponins from quinoa bran was 2.370% ± 0.022%.α-Glucosidase inhibitory activity of the extract was stronger than that of acarbose (P < 0.05),and the IC50 value was 0.620 ± 0.057 mg/mL.Enzyme kinetics study showed that total saponins extract of quinoa bran was a reversible mixed inhibition type.This study provides a reference for clarifying the mechanism of quinoa bran extract in reducing postprandial blood glucose and a basis for the development and utilization of quinoa bran.

Key words: total saponins from the bran of Chenopodium quinoa Willd.;response surface method;extraction technology, α-glucosidase;inhibition mechanism

中图分类号:  TQ461