天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (6): 971-976.doi: 10.16333/j.1001-6880.2021.6.010

• 研究简报 • 上一篇    下一篇

曲霉属真菌Aspergillus clavatonanicus HNMF114的次生代谢产物及其抗菌活性研究

王府润1,张圣良2,孔凡栋2,马青云2,谢晴宜2,戴好富3,陈萍1*,赵友兴2*   

  1. 1海南大学园艺学院,海口570228;2中国热带农业科学院热带生物技术研究所海口市热带天然产物研究与利用重点实验室;3中国热带农业科学院海南热带农业资源研究院,海口571101
  • 出版日期:2021-06-28 发布日期:2021-07-02
  • 基金资助:
    海南省自然科学基金创新团队项目(2019CXTD411);海南省自然科学基金(320RC491);农业农村部财政专项(NFZX2018);中国热带农业科学院基本科研业务费专项(17CXTD-15)

Secondary metabolites and their antibacterial activity from the marine fungus Aspergillus clavatonanicus HNMF114

WANG Fu-run1,ZHANG Sheng-liang2,KONG Fan-dong2, MA Qing-yun2,XIE Qing-yi2,DAI Hao-fu3,CHEN Ping1,*,ZHAO You-xing2,*   

  1. 1College of Horticulture,Hainan University,Haikou 570228,China;2Haikou Key Laboratory for Research and Utilization of Tropical Natural Products,Institute of Tropical Bioscience and Biotechnology,CATAS;3Hainan Academy of Tropical Agricultural Resource,CATAS,Haikou 571101,China

  • Online:2021-06-28 Published:2021-07-02

摘要:

本文对一株来自中华紫蛤的附生真菌HNMF114进行次生代谢产物的研究。从中分离鉴定了1个新化合物和8个已知化合物。分别鉴定为clavatone(1)、cyclo(L-Trp-L-Trp)(2)、2,4(1H,3H)-quinazolinedione (3)、clavatol(4)、kotanin(5)、orlandin(6)、desmethylkotanin(7)、(S)-2-(1-Hydroxy-2,2-dimethylpropyl)quinazolin-4(3H)-one(8)、(S)-2-methyl-1-(4-oxo-3,4- dihydroquinazolin-2-yl)propyl acetate(9)。新化合物1对大肠杆菌的生长具有一定的抑制作用,最小抑菌浓度(MIC)为2 μg/mL。化合物49对金黄色葡萄球菌有一定的抑制作用,最小抑制浓度分别为1 μg/mL和4 μg/mL。化合物5对枯草芽孢杆菌有一定的抑制作用,最小抑制浓度分别为8 μg/mL。化合物459对李斯特菌有一定的抑制作用,最小抑制浓度分别为4、4和8 μg/mL。

关键词: Aspergillus clavatonanicus, 次生代谢产物, 抗菌活性

Abstract:

The secondary metabolites of an endophytic fungus HNMF114 from the bivalve mollusk Sanguinolaria chinensis of China were studied in this paper.One new compound and eight known compounds were isolated and purified from the epiphytic fungus Aspergillus clavatonanicus,and the structures were identified as clavatone (1),cyclo(L-Trp-L-Trp) (2),2,4(1H,3H)-quinazolinedione (3),clavatol (4),kotanin (5),orlandin (6),desmethylkotanin (7),(S)-2-(1-hydroxy-2,2-dimethylpropyl)quinazolin-4(3H)-one (8),(S)-2-methyl-1-(4-oxo-3,4-dihydroquinazolin-2-yl) propyl acetate (9).The new compound 1 showed obvious inhibitory activity against Escherichia coli with the minimum inhibitory concentration (MIC) value of 2 μg/mL.Compounds 4 and 9 showed obvious inhibitory activity against Staphylococcus aureus with the MIC values of 1 μg/mL and 4 μg/mL,respectively.Compound 5 showed moderate inhibitory activity against Bacillus subtilis with the MIC value of 8 μg/mL.Compounds 4,5 and 9 showed moderate inhibitory activity against Listeria monocytogenes,with MIC values of 4,4 and 8 μg/mL,respectively.

Key words: Aspergillus clavatonanicus, secondary metabolites, antibacterial activity

中图分类号:  R284