天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (6): 977-981.doi: 10.16333/j.1001-6880.2021.6.011

• 研究简报 • 上一篇    下一篇

博落回茎中抗玉米大斑病的生物碱类成分研究

刘友花*,李健,王丹,解福双,郭志刚   

  1. 浙江清华长三角研究院,嘉兴 314000
  • 出版日期:2021-06-28 发布日期:2021-07-02

Alkaloids from the stem of Macleaya cordata against Exserohilum turcicum

LIU You-hua*,LI Jian,WANG Dan,XIE Fu-shuang,GUO Zhi-gang   

  1. Yangtze Delta Region Institute of Tsinghua University,Jiaxing 314000,China
  • Online:2021-06-28 Published:2021-07-02

摘要:

为了解博落回茎中具有抗玉米大斑病的活性成分,采用天然产物分离和活性追踪的方法分离鉴定了8个生物碱类化合物。这8个化合物分别为6-(2-羟基乙基)-5,6-二氢血根碱(1)、6-(2-甲氧基-2-氧代乙基)-5,6-二氢血根碱(2)、6-丙酮基二氢血根碱(3)、6-丙酮基白屈菜红碱(4)、isointegriamide(5)、arnottianamide(6)、二氢血根碱(7)和白屈菜红碱(8)。其中化合物1、2、5、6首次从博落回茎中分离得到。以上生物碱类化合物(1~8)通过滤纸片琼脂糖扩散法对玉米大斑病进行抗菌测试,确定了化合物8具有显著的抗玉米大斑病的生物活性,然后用48孔板测定了化合物8对玉米大斑病孢子萌发实验,其IC50值为12.0 μg/mL,以上研究为防治玉米大斑病提供了一种潜在的新型生物农药原料。

关键词: 博落回, 生物碱, 玉米大斑病, 活性成分

Abstract:

Eight alkaloids were isolated and identified by natural product separation and activity tracking in order to find out the active components in Macleaya cordata that were resistant to Exserohilum turcicum.The eight alkaloids were 6-(2-hydroxyethyl)-5,6-dihydrosanguinarine (1),6-(2-methoxy-2-oxyethyl)-5,6-dihydrosanguinarine (2),6-acetodihydrosanguinarine (3),6-acetonyldihydrochelerythrine (4),isointegriamide (5),arnottianamide (6),dihydrosanguinarine (7) and chelerythrine (8).Compounds 1,2,5,6 were isolated from the stem of M. cordata for the first time.The above alkaloid compounds (1-8) were tested by agar diffusion method with filter paper.It was confirmed that compound 8 had significant biological activity against E. turcicum.Then,the IC50 value of compound 8 on spore germination of E. turcicum was 12.0 μg/mL.From these results,our understanding of antifungal activity of chelerythrine against E. turcicum was enriched and this research is relevant for developing novel pesticides.

Key words: Macleaya cordata, alkaloids, Exserohilum turcicum, active components

中图分类号:  R932