To explore the potential therapeutic mechanism of Yunshi Ganmao Mixture on cold by using network pharmacological research method.TCMSP platform and literature search were used to screen the active chemical constituents and target genes of Yunshi Ganmao Mixture,and Uniprot was used to annotate the target genes.GeneCards databases and OMIM databases were used to search for cold-related disease targets.R (3.6.2) software was used to obtain the common target genes of chemical active components of cold and.Cytoscape 3.7.1 software was used to construct the active ingredient-influenza disease-target gene network of Yunshi Ganmao Mixture.The interaction between proteins corresponding to the target gene was evaluated using the STRING database.The Biocmanager package in R language was used for GO analysis,and the pathway analysis of common target genes was carried out using Reactome database.Results A total of 21 active components were screened out,including 197 target genes,3 715 cold-related genes and 152 intersection genes,such as AKT1,IL-6,ALB,VEGFA,CASP3.GO functional enrichment analysis revealed 159 entries and 398 Reactome pathways (P<0.05),mainly involving signal transfer to the immune system and a variety of diseases,such as PI3K/Akt signal,etc.Yunshi Ganmao Mixture plays a role in the treatment of cold through multi-component and multi-target therapy,mainly by regulating a variety of signal transduction pathways,as well as inflammatory immune response,and plays a role in regulating immunity and reducing inflammation.