天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (4): 630-638.doi: 10.16333/j.1001-6880.2022.4.011

• 开发研究 • 上一篇    下一篇

酶辅助超声法提取陕产黄精总黄酮及其抗氧化、抗A549细胞增殖活性研究

郭凯丽1，刘继平2，赵重博1，史永恒2，王   斌2，权利娜3，过晓芳4，朱星枚1*   

  1. 1陕西中医药大学药学院；2陕西省中医药管理局中药药效机制与物质基础重点研究室；3陕西省中药基础与新药研究重点实验室，咸阳 712046；4西安工业大学数理系，西安 710032
  • 出版日期:2022-04-29 发布日期:2022-04-29
  • 基金资助:
    国家自然科学基金（62102304）；陕西省教育厅重点实验室项目（21JS015）；陕西中医药大学研究生创新项目（2020CX33）；陕西中医药大学创新团队项目（2019-YL13）

Enzymatic-ultrasonic assisted extraction of total flavonoids from Shaanxi Polygonatum sibiricum and in vitro evaluation of their anti-oxidant and anti-A549 proliferation activities 

GUO Kai-li1,LIU Ji-ping2,ZHAO Chong-bo1,SHI Yong-heng2,WANG Bin2,QUAN Li-na3,GUO Xiao-fang4,ZHU Xing-mei1*   

  1. 1Department of Pharmacology,Shaanxi University of Chinese Medicine;2Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine;3Shaanxi Key Laboratory of Traditional Medicine Foundation and New Drug Research,Xianyang 712046,China;4School of Science,Xi'an Technological University,Xi'an 710021,China
  • Online:2022-04-29 Published:2022-04-29

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摘要：

优化陕产黄精总黄酮提取工艺，体外考查黄精总黄酮的抗氧化、抗A549细胞增殖活性。以黄精总黄酮含量为指标，在单因素试验基础上，应用Box-Behnken响应面法优化陕产黄精总黄酮提取工艺，黄精总黄酮最优提取工艺：2%纤维素酶，90%乙醇，超声20 min，料液比1∶16（g/mL），该工艺下黄精总黄酮的得率为103.10±0.02 μg/g，该法简便可行、重复性高。高效液相色谱（HPLC）法分析陕产黄精总黄酮，其中含槲皮素二水物6.43±0.25 μg/g和黄芩素92.31±1.75 μg/g。黄精总黄酮清除DPPH自由基、羟自由基和ABTS的EC50分别是11.20、57.88 和23.75 μg/mL，说明黄精总黄酮具有较强的抗氧化能力。CCK-8法表明黄精总黄酮作用A549细胞 24、48、72 h的IC50分别是25.18、12.52和9.25 μg/mL，黄精总黄酮可剂量和时间依赖性抗A549细胞增殖。

关键词: 黄精, 总黄酮, 酶辅助超声, 高效液相, 抗氧化, 抗增殖

Abstract:

The aim of this work was to investigate enzyme-assisted ultrasonic extraction of Shaanxi Polygonatum sibiricum and their anti-oxidant and anti-A549 proliferation activities in vitro.Based on the single factor test,Box-Behnken experimental was adopted including dependent variables(cellulase amount,ethanol concentration,ultrasonic time and solid-liquid ratio) and independent variable(yield of flavonoids).The results showed that the optimum conditions were cellulase amount 2%,ethanol concentration 90%,ultrasonic time 20 min,and solid-liquid ratio 1∶16(g/mL).The yield of total flavonoids reached 103.10 ± 0.02 μg/g.The method was simple,feasible and repeatable.High performance liquid chromatography(HPLC) analysis showed that the flavonoids contained 6.43±0.25 μg/g quercetin dihydrate and 92.31±1.75 μg/g baicalin.The EC50 of total flavonoids on scavenging DPPH free radical,hydroxyl free radical and ABTS were 11.20,57.88 and 23.75 μg/mL respectively,which indicated that total flavonoids from P. sibiricum had strong anti-oxidant capacity.CCK-8 tests showed that the IC50 of total flavonoids treating on A549 cells was 25.18,12.52 and 9.25 μg/mL at 24,48 and 72 h respectively,which indicated that total flavonoids from P. sibiricum could inhibit the proliferation of A549 cells in a dose- and time-dependent manner.

Key words: Polygonatum sibiricum, total flavonoids, enzymatic-ultrasonic, HPLC, anti-oxidant, anti-proliferation 

中图分类号:  Q282.71

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